Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4002431..4003007 Replicon chromosome
Accession NZ_CP100525
Organism Escherichia coli strain LH13-b

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NLZ09_RS19580 Protein ID WP_001297643.1
Coordinates 4002720..4003007 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NLZ09_RS19575 Protein ID WP_000063156.1
Coordinates 4002431..4002733 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ09_RS19560 (3999027) 3999027..4001177 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NLZ09_RS19565 (4001227) 4001227..4001430 + 204 WP_000467859.1 YbdD/YjiX family protein -
NLZ09_RS19570 (4001441) 4001441..4002397 + 957 WP_001297640.1 GTPase -
NLZ09_RS19575 (4002431) 4002431..4002733 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NLZ09_RS19580 (4002720) 4002720..4003007 - 288 WP_001297643.1 BrnT family toxin Toxin
NLZ09_RS19585 (4003206) 4003206..4003903 - 698 Protein_3830 IS1 family transposase -
NLZ09_RS19590 (4003960) 4003960..4004055 + 96 Protein_3831 dienelactone hydrolase family protein -
NLZ09_RS19595 (4004201) 4004201..4005433 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
NLZ09_RS19600 (4005474) 4005474..4006754 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T250767 WP_001297643.1 NZ_CP100525:c4003007-4002720 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT250767 WP_000063156.1 NZ_CP100525:c4002733-4002431 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References