Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 835704..836358 Replicon chromosome
Accession NZ_CP100525
Organism Escherichia coli strain LH13-b

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NLZ09_RS04105 Protein ID WP_000244777.1
Coordinates 835951..836358 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NLZ09_RS04100 Protein ID WP_000354046.1
Coordinates 835704..835970 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ09_RS04075 (830873) 830873..831616 + 744 WP_000951964.1 SDR family oxidoreductase -
NLZ09_RS04080 (831673) 831673..833106 - 1434 WP_254541866.1 6-phospho-beta-glucosidase BglA -
NLZ09_RS04085 (833151) 833151..833462 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
NLZ09_RS04090 (833626) 833626..834285 + 660 WP_000250274.1 hemolysin III family protein -
NLZ09_RS04095 (834481) 834481..835461 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NLZ09_RS04100 (835704) 835704..835970 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NLZ09_RS04105 (835951) 835951..836358 + 408 WP_000244777.1 protein YgfX Toxin
NLZ09_RS04110 (836398) 836398..836919 - 522 WP_001055874.1 flavodoxin FldB -
NLZ09_RS04115 (837031) 837031..837927 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NLZ09_RS04120 (837952) 837952..838662 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NLZ09_RS04125 (838668) 838668..840401 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T250756 WP_000244777.1 NZ_CP100525:835951-836358 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT250756 WP_000354046.1 NZ_CP100525:835704-835970 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References