Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 508884..509512 Replicon chromosome
Accession NC_014964
Organism Thermoanaerobacter brockii subsp. finnii Ako-1

Toxin (Protein)


Gene name mazF Uniprot ID B0KD57
Locus tag THEBR_RS02530 Protein ID WP_003867472.1
Coordinates 509162..509512 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID B0KD56
Locus tag THEBR_RS02525 Protein ID WP_003867471.1
Coordinates 508884..509159 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
THEBR_RS02500 504212..504577 + 366 WP_003867466.1 hypothetical protein -
THEBR_RS02505 504673..505089 + 417 WP_003867467.1 holo-ACP synthase -
THEBR_RS02510 505052..506584 + 1533 WP_013570786.1 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase -
THEBR_RS02515 506596..507606 + 1011 WP_003867469.1 outer membrane lipoprotein carrier protein LolA -
THEBR_RS02520 507628..508794 + 1167 WP_003867470.1 alanine racemase -
THEBR_RS02525 508884..509159 + 276 WP_003867471.1 ribbon-helix-helix protein, CopG family Antitoxin
THEBR_RS02530 509162..509512 + 351 WP_003867472.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
THEBR_RS02535 509840..510685 + 846 WP_003867473.1 YitT family protein -
THEBR_RS02540 510701..511522 + 822 WP_012268649.1 transketolase -
THEBR_RS02545 511523..512443 + 921 WP_012269028.1 transketolase family protein -
THEBR_RS02550 512472..512987 - 516 WP_012269029.1 hypothetical protein -
THEBR_RS02555 513171..514415 + 1245 WP_012269030.1 6-phosphofructokinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12933.02 Da        Isoelectric Point: 6.4714

>T25075 WP_003867472.1 NC_014964:509162-509512 [Thermoanaerobacter brockii subsp. finnii Ako-1]
MVIKRGDIFYADLSPVIGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHVEINGAEYGLNKDSVVLLEQI
RTIDKKRLREKIGHFDQEMMEKVNEALQISLGLIDF

Download         Length: 351 bp

>T25075 NC_014964:509162-509512 [Thermoanaerobacter brockii subsp. finnii Ako-1]
ATGGTGATAAAGAGGGGAGACATTTTTTATGCCGATTTAAGCCCTGTAATAGGCTCTGAACAGGGCGGAATTCGGCCTGT
ACTTATAATTCAAAATGATATTGGTAATAAATATAGTCCAACAGTAATAGTAGCGGCAATTACATCTCAGATTAACAAAG
CCAAATTGCCCACTCATGTTGAAATAAATGGGGCAGAATATGGACTTAACAAAGATTCTGTAGTATTATTGGAGCAAATA
AGGACTATTGACAAAAAACGTTTAAGAGAAAAAATTGGTCATTTTGACCAGGAAATGATGGAGAAAGTCAATGAAGCTTT
GCAAATAAGTTTAGGGTTGATTGATTTTTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10533.33 Da        Isoelectric Point: 6.3661

>AT25075 WP_003867471.1 NC_014964:508884-509159 [Thermoanaerobacter brockii subsp. finnii Ako-1]
VGETKRILVSLPQSLLEEVDVLAAMENRNRSEFIREAMKLYIREKKKIQIRESMKKGYLEMAAINSELAEMGLTAENECF
TGYEMKLKKCD

Download         Length: 276 bp

>AT25075 NC_014964:508884-509159 [Thermoanaerobacter brockii subsp. finnii Ako-1]
GTGGGCGAAACAAAGCGAATACTTGTAAGCTTGCCGCAAAGTTTATTAGAAGAAGTTGATGTTCTTGCCGCTATGGAAAA
CAGGAATCGCAGTGAATTTATAAGAGAAGCGATGAAATTATATATACGCGAGAAGAAAAAAATTCAAATACGAGAGAGCA
TGAAAAAGGGATATCTTGAGATGGCAGCAATTAACAGTGAACTTGCGGAAATGGGCCTAACCGCAGAAAACGAATGTTTT
ACAGGATATGAAATGAAATTGAAAAAGTGTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B3BRL3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M4W2H3

References