Detailed information of TA system TA250748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4797945..4799626
• Replicon: chromosome
• Accession: NZ_CP100520
• Organism: Klebsiella pneumoniae strain LH13-d

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: NLZ10_RS23280
• Protein ID: WP_004146663.1
• Coordinates: 4797945..4798862 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: NLZ10_RS23285
• Protein ID: WP_004177809.1
• Coordinates: 4798868..4799626 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NLZ10_RS23260 (4793251)	4793251..4794771	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
NLZ10_RS23265 (4794768)	4794768..4795766	+	999	WP_002885172.1	ribose ABC transporter permease	-
NLZ10_RS23270 (4795797)	4795797..4796738	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
NLZ10_RS23275 (4796813)	4796813..4797820	+	1008	WP_004221949.1	carbohydrate kinase family protein	-
NLZ10_RS23280 (4797945)	4797945..4798862	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
NLZ10_RS23285 (4798868)	4798868..4799626	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
NLZ10_RS23290 (4799712)	4799712..4800440	-	729	WP_002885152.1	response regulator transcription factor	-
NLZ10_RS23295 (4800713)	4800713..4802860	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
NLZ10_RS23300 (4803090)	4803090..4803776	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T250748 WP_004146663.1 NZ_CP100520:c4798862-4797945 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT250748 WP_004177809.1 NZ_CP100520:c4799626-4798868 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05