Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5248806..5249431 Replicon chromosome
Accession NZ_CP100515
Organism Klebsiella pneumoniae strain LH35-d

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NLZ11_RS25675 Protein ID WP_002882817.1
Coordinates 5248806..5249189 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NLZ11_RS25680 Protein ID WP_004150355.1
Coordinates 5249189..5249431 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ11_RS25660 (NLZ11_25660) 5246172..5247074 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NLZ11_RS25665 (NLZ11_25665) 5247071..5247706 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NLZ11_RS25670 (NLZ11_25670) 5247703..5248632 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NLZ11_RS25675 (NLZ11_25675) 5248806..5249189 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NLZ11_RS25680 (NLZ11_25680) 5249189..5249431 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NLZ11_RS25685 (NLZ11_25685) 5249636..5250553 + 918 WP_004178029.1 alpha/beta hydrolase -
NLZ11_RS25690 (NLZ11_25690) 5250567..5251508 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NLZ11_RS25695 (NLZ11_25695) 5251553..5251990 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NLZ11_RS25700 (NLZ11_25700) 5251987..5252847 - 861 WP_004146232.1 virulence factor BrkB family protein -
NLZ11_RS25705 (NLZ11_25705) 5252841..5253440 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250736 WP_002882817.1 NZ_CP100515:c5249189-5248806 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250736 WP_004150355.1 NZ_CP100515:c5249431-5249189 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References