Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5279955..5280580 Replicon chromosome
Accession NZ_CP100511
Organism Klebsiella pneumoniae strain LH50-a

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NLZ12_RS25860 Protein ID WP_002882817.1
Coordinates 5279955..5280338 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NLZ12_RS25865 Protein ID WP_004150355.1
Coordinates 5280338..5280580 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ12_RS25845 (NLZ12_25845) 5277321..5278223 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NLZ12_RS25850 (NLZ12_25850) 5278220..5278855 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NLZ12_RS25855 (NLZ12_25855) 5278852..5279781 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NLZ12_RS25860 (NLZ12_25860) 5279955..5280338 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NLZ12_RS25865 (NLZ12_25865) 5280338..5280580 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NLZ12_RS25870 (NLZ12_25870) 5280785..5281702 + 918 WP_004178029.1 alpha/beta hydrolase -
NLZ12_RS25875 (NLZ12_25875) 5281716..5282657 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NLZ12_RS25880 (NLZ12_25880) 5282702..5283139 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NLZ12_RS25885 (NLZ12_25885) 5283136..5283996 - 861 WP_004146232.1 virulence factor BrkB family protein -
NLZ12_RS25890 (NLZ12_25890) 5283990..5284589 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250720 WP_002882817.1 NZ_CP100511:c5280338-5279955 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250720 WP_004150355.1 NZ_CP100511:c5280580-5280338 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References