Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 17768..18369 Replicon plasmid pLH50-c-C
Accession NZ_CP100503
Organism Escherichia coli strain LH50-c

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag NLZ13_RS28525 Protein ID WP_001216034.1
Coordinates 17989..18369 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag NLZ13_RS28520 Protein ID WP_001190712.1
Coordinates 17768..17989 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ13_RS28510 (NLZ13_28515) 14794..16032 - 1239 WP_204154746.1 restriction endonuclease subunit S -
NLZ13_RS28515 (NLZ13_28520) 16029..17585 - 1557 WP_254539448.1 type I restriction-modification system subunit M -
NLZ13_RS28520 (NLZ13_28525) 17768..17989 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
NLZ13_RS28525 (NLZ13_28530) 17989..18369 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
NLZ13_RS28530 (NLZ13_28535) 18374..18553 + 180 WP_001513661.1 hypothetical protein -
NLZ13_RS28535 (NLZ13_28540) 18581..19624 + 1044 WP_001561086.1 DUF968 domain-containing protein -
NLZ13_RS28540 (NLZ13_28545) 19713..20165 + 453 WP_032165150.1 Late promoter-activating protein -
NLZ13_RS28545 (NLZ13_28550) 20251..21444 + 1194 WP_000219616.1 hypothetical protein -
NLZ13_RS28550 (NLZ13_28555) 21486..22928 + 1443 WP_254539449.1 terminase -
NLZ13_RS28555 (NLZ13_28560) 23140..23241 + 102 Protein_25 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..64795 64795


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T250700 WP_001216034.1 NZ_CP100503:17989-18369 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT250700 WP_001190712.1 NZ_CP100503:17768-17989 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References