250694

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 49396..50039 Replicon plasmid pLH50-c-A
Accession NZ_CP100501
Organism Escherichia coli strain LH50-c

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag NLZ13_RS27275 Protein ID WP_001044768.1
Coordinates 49396..49812 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag NLZ13_RS27280 Protein ID WP_001261287.1
Coordinates 49809..50039 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ13_RS27250 (NLZ13_27255) 44543..44827 + 285 WP_012006537.1 ribbon-helix-helix domain-containing protein -
NLZ13_RS27255 (NLZ13_27260) 44827..45102 + 276 WP_000421266.1 type II toxin-antitoxin system RelE/ParE family toxin -
NLZ13_RS27260 (NLZ13_27265) 45208..45447 + 240 WP_001105068.1 hypothetical protein -
NLZ13_RS27265 (NLZ13_27270) 45610..46782 - 1173 WP_062893848.1 IS91 family transposase -
NLZ13_RS27270 (NLZ13_27275) 47140..49234 + 2095 Protein_49 AAA family ATPase -
NLZ13_RS27275 (NLZ13_27280) 49396..49812 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
NLZ13_RS27280 (NLZ13_27285) 49809..50039 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NLZ13_RS27285 (NLZ13_27290) 50599..51012 + 414 WP_062893835.1 hypothetical protein -
NLZ13_RS27290 (NLZ13_27295) 51014..51799 + 786 WP_062893836.1 site-specific integrase -
NLZ13_RS27295 (NLZ13_27300) 52401..53033 + 633 WP_000312331.1 ParA family protein -
NLZ13_RS27300 (NLZ13_27305) 53033..53407 + 375 WP_000752652.1 hypothetical protein -
NLZ13_RS27305 (NLZ13_27310) 53646..54620 + 975 WP_001217833.1 plasmid segregation protein ParM -
NLZ13_RS27310 (NLZ13_27315) 54624..55016 + 393 WP_112038214.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..139970 139970
- inside IScluster/Tn - - 30364..46902 16538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T250694 WP_001044768.1 NZ_CP100501:c49812-49396 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT250694 WP_001261287.1 NZ_CP100501:c50039-49809 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References