250693

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 44543..45102 Replicon plasmid pLH50-c-A
Accession NZ_CP100501
Organism Escherichia coli strain LH50-c

Toxin (Protein)


Gene name relE Uniprot ID A0A0H2XJR9
Locus tag NLZ13_RS27255 Protein ID WP_000421266.1
Coordinates 44827..45102 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0H2XL20
Locus tag NLZ13_RS27250 Protein ID WP_012006537.1
Coordinates 44543..44827 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ13_RS27225 (NLZ13_27230) 40381..41062 - 682 Protein_40 ABC transporter ATP-binding protein -
NLZ13_RS27230 (NLZ13_27235) 41062..42099 - 1038 WP_000110580.1 iron ABC transporter permease -
NLZ13_RS27235 (NLZ13_27240) 42099..43097 - 999 WP_000756335.1 ABC transporter substrate-binding protein -
NLZ13_RS27240 (NLZ13_27245) 43247..43405 - 159 Protein_43 integrase core domain-containing protein -
NLZ13_RS27245 (NLZ13_27250) 43452..44042 - 591 WP_000086535.1 recombinase family protein -
NLZ13_RS27250 (NLZ13_27255) 44543..44827 + 285 WP_012006537.1 ribbon-helix-helix domain-containing protein Antitoxin
NLZ13_RS27255 (NLZ13_27260) 44827..45102 + 276 WP_000421266.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NLZ13_RS27260 (NLZ13_27265) 45208..45447 + 240 WP_001105068.1 hypothetical protein -
NLZ13_RS27265 (NLZ13_27270) 45610..46782 - 1173 WP_062893848.1 IS91 family transposase -
NLZ13_RS27270 (NLZ13_27275) 47140..49234 + 2095 Protein_49 AAA family ATPase -
NLZ13_RS27275 (NLZ13_27280) 49396..49812 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin -
NLZ13_RS27280 (NLZ13_27285) 49809..50039 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..139970 139970
- inside IScluster/Tn - - 30364..46902 16538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10779.47 Da        Isoelectric Point: 9.9324

>T250693 WP_000421266.1 NZ_CP100501:44827-45102 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAATRTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10501.96 Da        Isoelectric Point: 6.4632

>AT250693 WP_012006537.1 NZ_CP100501:44543-44827 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVKSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2XJR9

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2XL20

References