Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5053521..5054018 Replicon chromosome
Accession NZ_CP100500
Organism Escherichia coli strain LH50-c

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NLZ13_RS24680 Protein ID WP_001173343.1
Coordinates 5053521..5053694 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NLZ13_RS24685 Protein ID WP_000398619.1
Coordinates 5053722..5054018 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ13_RS24670 (5050191) 5050191..5051648 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
NLZ13_RS24675 (5051885) 5051885..5053402 + 1518 WP_001295065.1 lysine--tRNA ligase -
NLZ13_RS24680 (5053521) 5053521..5053694 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NLZ13_RS24685 (5053722) 5053722..5054018 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NLZ13_RS24690 (5054245) 5054245..5054517 - 273 WP_000405653.1 GNAT family N-acetyltransferase -
NLZ13_RS24695 (5054529) 5054529..5054759 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NLZ13_RS24700 (5054940) 5054940..5056571 + 1632 WP_001216470.1 sensor histidine kinase -
NLZ13_RS24705 (5056568) 5056568..5057287 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5028216..5054018 25802


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T250690 WP_001173343.1 NZ_CP100500:c5053694-5053521 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT250690 WP_000398619.1 NZ_CP100500:c5054018-5053722 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References