Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4732679..4733255 Replicon chromosome
Accession NZ_CP100500
Organism Escherichia coli strain LH50-c

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag NLZ13_RS23230 Protein ID WP_001295743.1
Coordinates 4732968..4733255 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag NLZ13_RS23225 Protein ID WP_000063148.1
Coordinates 4732679..4732981 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ13_RS23205 (4728440) 4728440..4728982 + 543 WP_000934391.1 hypothetical protein -
NLZ13_RS23210 (4729275) 4729275..4731425 + 2151 WP_254539341.1 pyruvate/proton symporter BtsT -
NLZ13_RS23215 (4731475) 4731475..4731678 + 204 WP_000467859.1 YbdD/YjiX family protein -
NLZ13_RS23220 (4731689) 4731689..4732645 + 957 WP_089625875.1 GTPase -
NLZ13_RS23225 (4732679) 4732679..4732981 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
NLZ13_RS23230 (4732968) 4732968..4733255 - 288 WP_001295743.1 BrnT family toxin Toxin
NLZ13_RS23235 (4733452) 4733452..4734618 + 1167 WP_000800831.1 restriction endonuclease -
NLZ13_RS23240 (4734682) 4734682..4736301 + 1620 WP_089625877.1 class I SAM-dependent DNA methyltransferase -
NLZ13_RS23245 (4736291) 4736291..4737604 + 1314 WP_112037750.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T250687 WP_001295743.1 NZ_CP100500:c4733255-4732968 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT250687 WP_000063148.1 NZ_CP100500:c4732981-4732679 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References