Detailed information of TA system TA025068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1660898..1661487
• Replicon: chromosome
• Accession: NC_014934
• Organism: Cellulophaga algicola DSM 14237

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: E6X9K6
• Locus tag: CELAL_RS07180
• Protein ID: WP_013550238.1
• Coordinates: 1660898..1661197 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E6X9K7
• Locus tag: CELAL_RS07185
• Protein ID: WP_013550239.1
• Coordinates: 1661203..1661487 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CELAL_RS07145	1656088..1656567	+	480	WP_013550233.1	hypothetical protein	-
CELAL_RS07150	1656628..1657299	+	672	WP_013550234.1	hypothetical protein	-
CELAL_RS07155	1657524..1657838	+	315	WP_013550235.1	hypothetical protein	-
CELAL_RS07160	1658063..1658908	+	846	WP_052303984.1	toxin-antitoxin system YwqK family antitoxin	-
CELAL_RS07170	1659509..1659751	+	243	WP_013550237.1	hypothetical protein	-
CELAL_RS07180	1660898..1661197	+	300	WP_013550238.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CELAL_RS07185	1661203..1661487	+	285	WP_013550239.1	putative addiction module antidote protein	Antitoxin
CELAL_RS07190	1661704..1662465	+	762	WP_041557597.1	YARHG domain-containing protein	-
CELAL_RS21840	1663234..1664511	+	1278	WP_013550241.1	DUF4236 domain-containing protein	-
CELAL_RS21415	1665309..1665818	+	510	WP_013550242.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1665868..1666761	893

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-92)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11693.75 Da
• Isoelectric Point: 10.4228

>T25068 WP_013550238.1 NC_014934:1660898-1661197 [Cellulophaga algicola DSM 14237]
MFFIEKTIEFDKWLRKLKDLKGKAKILFRIQKLESDEHFGDCKSVGDGIRELRVNFAKGYRVYFKEKDGKIIVLLIGGDK
STQQQDIKKAKEIWNKLNK

Nucleotide

• Length: 300 bp

>T25068 NC_014934:1660898-1661197 [Cellulophaga algicola DSM 14237]
ATGTTCTTTATTGAGAAAACAATCGAATTTGATAAGTGGCTTAGAAAGTTAAAAGACTTGAAAGGCAAGGCAAAGATTCT
GTTTAGGATTCAGAAACTTGAGAGCGACGAACATTTTGGAGATTGTAAATCTGTTGGAGACGGAATTAGGGAATTAAGAG
TAAATTTTGCCAAAGGTTACAGAGTTTACTTCAAAGAAAAAGATGGAAAAATAATTGTTCTACTAATTGGTGGAGATAAG
TCGACCCAACAACAAGACATAAAAAAAGCGAAAGAAATCTGGAACAAATTAAATAAATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10063.56 Da
• Isoelectric Point: 4.7205

>AT25068 WP_013550239.1 NC_014934:1661203-1661487 [Cellulophaga algicola DSM 14237]
MGTSKFDIADYLDSKEMIAEYLNTVLEEGDNADVINAIGHIAKAIGMTKISEETGLSRPSLYKALSDGSKPQFATIMKVL
KAIGGQIQVNPMSA

Nucleotide

• Length: 285 bp

>AT25068 NC_014934:1661203-1661487 [Cellulophaga algicola DSM 14237]
ATGGGAACTTCAAAATTTGACATAGCGGATTATTTGGACAGCAAAGAAATGATTGCAGAGTACCTCAATACTGTTTTAGA
AGAAGGAGATAATGCAGATGTGATCAATGCAATTGGACACATTGCAAAAGCGATTGGAATGACCAAGATATCTGAAGAAA
CTGGATTAAGTAGACCGAGTTTGTACAAAGCGTTATCAGATGGTTCTAAACCTCAATTCGCTACGATTATGAAAGTTTTG
AAAGCGATTGGAGGACAAATTCAGGTCAATCCAATGTCTGCCTAA

Structures

Toxin

• AlphaFold DB: E6X9K6

Antitoxin

• AlphaFold DB: E6X9K7