250673

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1276538..1277121 Replicon chromosome
Accession NZ_CP100500
Organism Escherichia coli strain LH50-c

Toxin (Protein)


Gene name mazF Uniprot ID U9Y5H3
Locus tag NLZ13_RS06245 Protein ID WP_000254735.1
Coordinates 1276786..1277121 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U9Y5F1
Locus tag NLZ13_RS06240 Protein ID WP_000581936.1
Coordinates 1276538..1276786 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ13_RS06230 (1272877) 1272877..1274178 + 1302 WP_000046797.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
NLZ13_RS06235 (1274226) 1274226..1276460 + 2235 WP_000226815.1 GTP pyrophosphokinase -
NLZ13_RS06240 (1276538) 1276538..1276786 + 249 WP_000581936.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
NLZ13_RS06245 (1276786) 1276786..1277121 + 336 WP_000254735.1 endoribonuclease MazF Toxin
NLZ13_RS06250 (1277193) 1277193..1277984 + 792 WP_001071673.1 nucleoside triphosphate pyrophosphohydrolase -
NLZ13_RS06255 (1278212) 1278212..1279849 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
NLZ13_RS06260 (1279937) 1279937..1281235 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12070.03 Da        Isoelectric Point: 8.2605

>T250673 WP_000254735.1 NZ_CP100500:1276786-1277121 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRAKGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9341.71 Da        Isoelectric Point: 4.4536

>AT250673 WP_000581936.1 NZ_CP100500:1276538-1276786 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHDNIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Y5H3

Antitoxin

Source ID Structure
AlphaFold DB U9Y5F1

References