Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 50282..51018 Replicon plasmid pLH71-A
Accession NZ_CP100498
Organism Klebsiella michiganensis strain LH71

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag NLZ14_RS27820 Protein ID WP_004098919.1
Coordinates 50536..51018 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A2J5Q9C0
Locus tag NLZ14_RS27815 Protein ID WP_004098921.1
Coordinates 50282..50548 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ14_RS27785 (NLZ14_27785) 45626..46201 + 576 WP_044348232.1 TerD family protein -
NLZ14_RS27790 (NLZ14_27790) 46289..47530 + 1242 WP_047727579.1 VWA domain-containing protein -
NLZ14_RS27795 (NLZ14_27795) 47558..48208 - 651 WP_044348228.1 HAD family hydrolase -
NLZ14_RS27805 (NLZ14_27805) 49613..49837 + 225 WP_254540122.1 hypothetical protein -
NLZ14_RS27810 (NLZ14_27810) 49881..50075 - 195 WP_110188634.1 hypothetical protein -
NLZ14_RS27815 (NLZ14_27815) 50282..50548 + 267 WP_004098921.1 DUF1778 domain-containing protein Antitoxin
NLZ14_RS27820 (NLZ14_27820) 50536..51018 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
NLZ14_RS27825 (NLZ14_27825) 51177..52697 - 1521 WP_254540123.1 IS3 family transposase -
NLZ14_RS27830 (NLZ14_27830) 52753..53201 + 449 Protein_56 IS1 family transposase -
NLZ14_RS27835 (NLZ14_27835) 53646..54215 + 570 WP_254540124.1 fimbrial protein -
NLZ14_RS27840 (NLZ14_27840) 54274..54969 + 696 WP_254540125.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..125674 125674
- inside IScluster/Tn - - 48428..53201 4773


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T250664 WP_004098919.1 NZ_CP100498:50536-51018 [Klebsiella michiganensis]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10105.49 Da        Isoelectric Point: 4.5141

>AT250664 WP_004098921.1 NZ_CP100498:50282-50548 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q9C0

References