250662

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5132234..5132810 Replicon chromosome
Accession NZ_CP100497
Organism Klebsiella michiganensis strain LH71

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag NLZ14_RS24045 Protein ID WP_254540085.1
Coordinates 5132523..5132810 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0H3H591
Locus tag NLZ14_RS24040 Protein ID WP_014227757.1
Coordinates 5132234..5132536 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ14_RS24025 (NLZ14_24025) 5129070..5129405 + 336 WP_064375703.1 endoribonuclease SymE -
NLZ14_RS24030 (NLZ14_24030) 5129862..5130773 + 912 WP_254539709.1 acetamidase/formamidase family protein -
NLZ14_RS24035 (NLZ14_24035) 5130770..5132113 + 1344 WP_148849614.1 APC family permease -
NLZ14_RS24040 (NLZ14_24040) 5132234..5132536 - 303 WP_014227757.1 BrnA antitoxin family protein Antitoxin
NLZ14_RS24045 (NLZ14_24045) 5132523..5132810 - 288 WP_254540085.1 BrnT family toxin Toxin
NLZ14_RS24050 (NLZ14_24050) 5133070..5133513 - 444 WP_064375701.1 FosA family fosfomycin resistance glutathione transferase -
NLZ14_RS24055 (NLZ14_24055) 5133507..5134415 - 909 WP_221842232.1 LysR family transcriptional regulator -
NLZ14_RS24060 (NLZ14_24060) 5134503..5135285 + 783 WP_049083709.1 NAD(P)H-dependent oxidoreductase -
NLZ14_RS24065 (NLZ14_24065) 5135433..5136017 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
NLZ14_RS24070 (NLZ14_24070) 5136163..5136963 + 801 WP_254539710.1 winged helix-turn-helix domain-containing protein -
NLZ14_RS24075 (NLZ14_24075) 5136960..5137478 + 519 WP_004098240.1 FidL-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11226.69 Da        Isoelectric Point: 7.4687

>T250662 WP_254540085.1 NZ_CP100497:c5132810-5132523 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKSDSKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11607.18 Da        Isoelectric Point: 9.4429

>AT250662 WP_014227757.1 NZ_CP100497:c5132536-5132234 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H591

References