Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2007910..2008500 Replicon chromosome
Accession NZ_CP100497
Organism Klebsiella michiganensis strain LH71

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag NLZ14_RS09535 Protein ID WP_014229979.1
Coordinates 2008168..2008500 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag NLZ14_RS09530 Protein ID WP_004852307.1
Coordinates 2007910..2008167 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ14_RS09505 (NLZ14_09505) 2003155..2003772 - 618 WP_128334957.1 glutathione S-transferase -
NLZ14_RS09510 (NLZ14_09510) 2003952..2004878 + 927 WP_049101399.1 LysR substrate-binding domain-containing protein -
NLZ14_RS09515 (NLZ14_09515) 2004916..2005914 - 999 WP_148841994.1 aldo/keto reductase -
NLZ14_RS09520 (NLZ14_09520) 2006015..2006929 + 915 WP_025106070.1 LysR family transcriptional regulator -
NLZ14_RS09525 (NLZ14_09525) 2007048..2007317 - 270 WP_064391807.1 hypothetical protein -
NLZ14_RS09530 (NLZ14_09530) 2007910..2008167 + 258 WP_004852307.1 antitoxin Antitoxin
NLZ14_RS09535 (NLZ14_09535) 2008168..2008500 + 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NLZ14_RS09540 (NLZ14_09540) 2008827..2010065 + 1239 WP_254539943.1 hypothetical protein -
NLZ14_RS09545 (NLZ14_09545) 2010235..2011959 - 1725 WP_227634498.1 response regulator receiver domain -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T250655 WP_014229979.1 NZ_CP100497:2008168-2008500 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT250655 WP_004852307.1 NZ_CP100497:2007910-2008167 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References