Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56959..57506 Replicon chromosome
Accession NZ_CP100497
Organism Klebsiella michiganensis strain LH71

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag NLZ14_RS00290 Protein ID WP_014227299.1
Coordinates 56959..57267 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag NLZ14_RS00295 Protein ID WP_014227298.1
Coordinates 57270..57506 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ14_RS00260 (NLZ14_00260) 52606..52917 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
NLZ14_RS00265 (NLZ14_00265) 53212..54153 + 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
NLZ14_RS00270 (NLZ14_00270) 54177..54470 - 294 WP_014227302.1 YicS family protein -
NLZ14_RS00275 (NLZ14_00275) 54680..55645 + 966 WP_064377595.1 Rpn family recombination-promoting nuclease/putative transposase -
NLZ14_RS00280 (NLZ14_00280) 55645..55902 + 258 WP_032718834.1 hypothetical protein -
NLZ14_RS00285 (NLZ14_00285) 55906..56808 - 903 WP_014227300.1 EamA family transporter -
NLZ14_RS00290 (NLZ14_00290) 56959..57267 - 309 WP_014227299.1 CcdB family protein Toxin
NLZ14_RS00295 (NLZ14_00295) 57270..57506 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
NLZ14_RS00300 (NLZ14_00300) 57612..59045 - 1434 WP_048262922.1 PTS N-acetylmuramic acid transporter subunit IIBC -
NLZ14_RS00305 (NLZ14_00305) 59070..59972 - 903 WP_032752002.1 N-acetylmuramic acid 6-phosphate etherase -
NLZ14_RS00310 (NLZ14_00310) 60133..61299 - 1167 WP_014227296.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T250652 WP_014227299.1 NZ_CP100497:c57267-56959 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT250652 WP_014227298.1 NZ_CP100497:c57506-57270 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References