Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 1830164..1830685 Replicon chromosome
Accession NC_014925
Organism Staphylococcus pseudintermedius HKU10-03

Toxin (Protein)


Gene name TsdT Uniprot ID A0A3D8YLE8
Locus tag SPSINT_RS08960 Protein ID WP_014613364.1
Coordinates 1830164..1830385 (-) Length 74 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A166R1D0
Locus tag SPSINT_RS08965 Protein ID WP_014613363.1
Coordinates 1830398..1830685 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SPSINT_RS08940 1825745..1826473 - 729 WP_014613368.1 hypothetical protein -
SPSINT_RS13050 1827441..1827587 - 147 WP_014613367.1 hypothetical protein -
SPSINT_RS08950 1827673..1829853 - 2181 WP_015729432.1 heavy metal translocating P-type ATPase -
SPSINT_RS08955 1829954..1830160 - 207 WP_014613365.1 cation transporter -
SPSINT_RS08960 1830164..1830385 - 222 WP_014613364.1 heavy-metal-associated domain-containing protein Toxin
SPSINT_RS08965 1830398..1830685 - 288 WP_014613363.1 copper-sensing transcriptional repressor CsoR Antitoxin
SPSINT_RS08970 1830817..1832307 + 1491 WP_014613362.1 cardiolipin synthase -
SPSINT_RS08975 1832328..1832984 + 657 WP_014613361.1 HD domain-containing protein -
SPSINT_RS08980 1833038..1833904 - 867 WP_015729433.1 membrane protein insertase YidC -
SPSINT_RS08985 1833993..1834631 - 639 WP_014613359.1 thiamine phosphate synthase -
SPSINT_RS08990 1834621..1835424 - 804 WP_014613358.1 hydroxyethylthiazole kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(21-64)

Antitoxin

Metal-sensitive transcriptional repressor

(3-93)


Sequences

Toxin        


Download         Length: 74 a.a.        Molecular weight: 8065.10 Da        Isoelectric Point: 4.3480

>T25064 WP_014613364.1 NC_014925:c1830385-1830164 [Staphylococcus pseudintermedius HKU10-03]
MENGYVKVTQLTTEAQQQQLSDRLLRLIGVESVAIDVATQSLTLTYDTPANLNTIEKEIYDAGFPVIQSHKGV

Download         Length: 222 bp

>T25064 NC_014925:c1830385-1830164 [Staphylococcus pseudintermedius HKU10-03]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10997.59 Da        Isoelectric Point: 7.0504

>AT25064 WP_014613363.1 NC_014925:c1830685-1830398 [Staphylococcus pseudintermedius HKU10-03]
MTEQAHHSEEIKRNLKSRLNRIEGQVKAINRMIDEDVYCDDVLTQIRATRSALNSVASRLLDHHMKGCIKHKIDAGHQDE
AMEELLVTFQKLMKD

Download         Length: 288 bp

>AT25064 NC_014925:c1830685-1830398 [Staphylococcus pseudintermedius HKU10-03]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3D8YLE8

Antitoxin

Source ID Structure
AlphaFold DB A0A166R1D0

References