Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4539042..4539539 Replicon chromosome
Accession NZ_CP100486
Organism Escherichia coli strain LH85-a

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NLZ16_RS21630 Protein ID WP_001173343.1
Coordinates 4539042..4539215 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NLZ16_RS21635 Protein ID WP_000398619.1
Coordinates 4539243..4539539 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ16_RS21620 (4535712) 4535712..4537169 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
NLZ16_RS21625 (4537406) 4537406..4538923 + 1518 WP_001550472.1 lysine--tRNA ligase -
NLZ16_RS21630 (4539042) 4539042..4539215 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NLZ16_RS21635 (4539243) 4539243..4539539 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NLZ16_RS21640 (4539768) 4539768..4540040 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NLZ16_RS21645 (4540052) 4540052..4540282 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
NLZ16_RS21650 (4540462) 4540462..4542093 + 1632 WP_001216477.1 sensor histidine kinase -
NLZ16_RS21655 (4542090) 4542090..4542809 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4504420..4539539 35119


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T250631 WP_001173343.1 NZ_CP100486:c4539215-4539042 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT250631 WP_000398619.1 NZ_CP100486:c4539539-4539243 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References