Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1815245..1815769 Replicon chromosome
Accession NC_014925
Organism Staphylococcus pseudintermedius HKU10-03

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag SPSINT_RS08885 Protein ID WP_014613379.1
Coordinates 1815245..1815598 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag SPSINT_RS08890 Protein ID WP_014613378.1
Coordinates 1815599..1815769 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SPSINT_RS08865 1812554..1813324 - 771 WP_015729428.1 RNA polymerase sigma factor SigB -
SPSINT_RS08870 1813299..1813778 - 480 WP_041614876.1 anti-sigma B factor RsbW -
SPSINT_RS08875 1813778..1814104 - 327 WP_014613381.1 anti-sigma factor antagonist -
SPSINT_RS08880 1814183..1815187 - 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
SPSINT_RS08885 1815245..1815598 - 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
SPSINT_RS08890 1815599..1815769 - 171 WP_014613378.1 hypothetical protein Antitoxin
SPSINT_RS08895 1815854..1817002 - 1149 WP_015729430.1 alanine racemase -
SPSINT_RS08900 1817036..1817386 - 351 WP_014613376.1 holo-ACP synthase -
SPSINT_RS08905 1817383..1817901 - 519 WP_014613375.1 PH domain-containing protein -
SPSINT_RS08910 1817885..1819414 - 1530 WP_014613374.1 PH domain-containing protein -
SPSINT_RS08915 1819407..1819880 - 474 WP_014613373.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T25063 WP_014613379.1 NC_014925:c1815598-1815245 [Staphylococcus pseudintermedius HKU10-03]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T25063 NC_014925:c1815598-1815245 [Staphylococcus pseudintermedius HKU10-03]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATTCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT25063 WP_014613378.1 NC_014925:c1815769-1815599 [Staphylococcus pseudintermedius HKU10-03]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT25063 NC_014925:c1815769-1815599 [Staphylococcus pseudintermedius HKU10-03]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References