Detailed information of TA system TA250626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3913641..3915330
• Replicon: chromosome
• Accession: NZ_CP100486
• Organism: Escherichia coli strain LH85-a

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: NLZ16_RS18660
• Protein ID: WP_000365809.1
• Coordinates: 3913641..3914561 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: NLZ16_RS18665
• Protein ID: WP_001550661.1
• Coordinates: 3914548..3915330 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NLZ16_RS18645 (3909731)	3909731..3910858	-	1128	WP_001550663.1	MFS transporter	-
NLZ16_RS18650 (3911000)	3911000..3912058	-	1059	WP_122987197.1	alpha/beta hydrolase	-
NLZ16_RS18655 (3912323)	3912323..3913207	+	885	WP_001119037.1	LysR family transcriptional regulator	-
NLZ16_RS18660 (3913641)	3913641..3914561	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
NLZ16_RS18665 (3914548)	3914548..3915330	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
NLZ16_RS18670 (3915646)	3915646..3918855	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
NLZ16_RS18675 (3919202)	3919202..3919672	+	471	WP_000201185.1	hypothetical protein	-
NLZ16_RS18680 (3919669)	3919669..3919771	+	103	Protein_3658	helix-turn-helix domain-containing protein	-
NLZ16_RS18685 (3919863)	3919863..3920021	+	159	Protein_3659	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T250626 WP_000365809.1 NZ_CP100486:c3914561-3913641 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT250626 WP_001550661.1 NZ_CP100486:c3915330-3914548 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: V0Z9M0