Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5122347..5122972 Replicon chromosome
Accession NZ_CP100478
Organism Klebsiella pneumoniae strain LH99-d

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NLZ18_RS24790 Protein ID WP_002882817.1
Coordinates 5122347..5122730 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NLZ18_RS24795 Protein ID WP_004150355.1
Coordinates 5122730..5122972 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ18_RS24775 (5119713) 5119713..5120615 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NLZ18_RS24780 (5120612) 5120612..5121247 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NLZ18_RS24785 (5121244) 5121244..5122173 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NLZ18_RS24790 (5122347) 5122347..5122730 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NLZ18_RS24795 (5122730) 5122730..5122972 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NLZ18_RS24800 (5123177) 5123177..5124094 + 918 WP_040238240.1 alpha/beta hydrolase -
NLZ18_RS24805 (5124108) 5124108..5125049 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NLZ18_RS24810 (5125094) 5125094..5125531 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NLZ18_RS24815 (5125528) 5125528..5126388 - 861 WP_002882807.1 virulence factor BrkB family protein -
NLZ18_RS24820 (5126382) 5126382..5126981 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250595 WP_002882817.1 NZ_CP100478:c5122730-5122347 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250595 WP_004150355.1 NZ_CP100478:c5122972-5122730 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References