Detailed information of TA system TA250594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4798233..4799914
• Replicon: chromosome
• Accession: NZ_CP100478
• Organism: Klebsiella pneumoniae strain LH99-d

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: NLZ18_RS23285
• Protein ID: WP_004146663.1
• Coordinates: 4798233..4799150 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: NLZ18_RS23290
• Protein ID: WP_004177809.1
• Coordinates: 4799156..4799914 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NLZ18_RS23265 (4793539)	4793539..4795059	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
NLZ18_RS23270 (4795056)	4795056..4796054	+	999	WP_002885172.1	ribose ABC transporter permease	-
NLZ18_RS23275 (4796085)	4796085..4797026	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
NLZ18_RS23280 (4797101)	4797101..4798108	+	1008	WP_004221949.1	carbohydrate kinase family protein	-
NLZ18_RS23285 (4798233)	4798233..4799150	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
NLZ18_RS23290 (4799156)	4799156..4799914	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
NLZ18_RS23295 (4800000)	4800000..4800728	-	729	WP_002885152.1	response regulator transcription factor	-
NLZ18_RS23300 (4801001)	4801001..4803148	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
NLZ18_RS23305 (4803378)	4803378..4804064	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T250594 WP_004146663.1 NZ_CP100478:c4799150-4798233 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT250594 WP_004177809.1 NZ_CP100478:c4799914-4799156 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05