Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4534242..4535052 Replicon chromosome
Accession NZ_CP100478
Organism Klebsiella pneumoniae strain LH99-d

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NLZ18_RS22050 Protein ID WP_004178461.1
Coordinates 4534242..4534775 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NLZ18_RS22055 Protein ID WP_002887278.1
Coordinates 4534786..4535052 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ18_RS22045 (4533073) 4533073..4534194 + 1122 WP_009309849.1 cupin domain-containing protein -
NLZ18_RS22050 (4534242) 4534242..4534775 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NLZ18_RS22055 (4534786) 4534786..4535052 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NLZ18_RS22060 (4535155) 4535155..4536588 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
NLZ18_RS22065 (4536578) 4536578..4537261 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NLZ18_RS22070 (4537434) 4537434..4538819 + 1386 WP_040238382.1 efflux transporter outer membrane subunit -
NLZ18_RS22075 (4538837) 4538837..4539181 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T250592 WP_004178461.1 NZ_CP100478:c4534775-4534242 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT250592 WP_002887278.1 NZ_CP100478:c4535052-4534786 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References