Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rlegTA/AbiEii-AbiEi
Location 2364580..2366062 Replicon chromosome
Accession NZ_CP100476
Organism Mesorhizobium sp. C120A

Toxin (Protein)


Gene name rlegT Uniprot ID V7HSE2
Locus tag NL532_RS11650 Protein ID WP_023828051.1
Coordinates 2365193..2366062 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name rlegA Uniprot ID V7HPU5
Locus tag NL532_RS11645 Protein ID WP_023828052.1
Coordinates 2364580..2365200 (+) Length 207 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL532_RS11635 (NL532_11620) 2362259..2363863 - 1605 WP_023828054.1 DNA (cytosine-5-)-methyltransferase -
NL532_RS11640 (NL532_11625) 2363940..2364446 + 507 WP_023828053.1 DNA mismatch endonuclease Vsr -
NL532_RS11645 (NL532_11630) 2364580..2365200 + 621 WP_023828052.1 AbiEi antitoxin N-terminal domain-containing protein Antitoxin
NL532_RS11650 (NL532_11635) 2365193..2366062 + 870 WP_023828051.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
NL532_RS11655 (NL532_11640) 2366227..2367777 + 1551 WP_023828050.1 restriction endonuclease -
NL532_RS11660 (NL532_11645) 2367990..2369270 - 1281 WP_023828049.1 hypothetical protein -
NL532_RS11665 (NL532_11650) 2369888..2370556 + 669 WP_023828048.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2342078..2411425 69347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-264)

Antitoxin

Transcriptional regulator AbiEi antitoxin, N-terminal domain

(7-92)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 31923.43 Da        Isoelectric Point: 5.7590

>T250578 WP_023828051.1 NZ_CP100476:2365193-2366062 [Mesorhizobium sp. C120A]
MARGTTNQAASIKQRLLNLARKEGRIYEVVLVRYALERMLYRLSVSDQRDRFILKGGMLVTLWIDGGNRETRDADFLGHG
DANPEKLKATFAEIMAIATDDGLVFDTQGLTATTIREEMEYGGVRLRTTAFLERSRIPVTIDIGFGDALAAAAQPLAYPS
LLGMETPQIRTYPPATVIAEKFQAMVALGIANGRMKDYYDLWAIPKAVPLADTDLDAAIRATFERRHTDIPDGRPPGLST
TLLDDATKQRQWHAYAQSIDHDGLSLVEVVDAVWALVGPSCARIRSSDN

Download         Length: 870 bp

Antitoxin


Download         Length: 207 a.a.        Molecular weight: 22932.43 Da        Isoelectric Point: 10.2816

>AT250578 WP_023828052.1 NZ_CP100476:2364580-2365200 [Mesorhizobium sp. C120A]
MDRTQTTQRQQLAALLDTQPIMRAYELRKAGIAPETISRAVHNGQLIRISRGLYQRADSEVETEQALAEAAKRVPKGVIA
MVSALAFHGLTDQMPRKIWVAISARDWAPVPSYPPIRIVELRDRYMDQGIEHHRISGVDVPIFSVPKTLADIFRNRKLVD
RSVAVEGLRAALDQRKATPGTIAEAAIAGDSWKIMRPYLEALTSNG

Download         Length: 621 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V7HSE2

Antitoxin

Source ID Structure
AlphaFold DB V7HPU5

References