Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 1108285..1108955 Replicon chromosome
Accession NZ_CP100472
Organism Mesorhizobium sp. C399B

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag NLY36_RS05330 Protein ID WP_023696188.1
Coordinates 1108285..1108683 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID X6J8K0
Locus tag NLY36_RS05335 Protein ID WP_023687970.1
Coordinates 1108683..1108955 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLY36_RS05305 (NLY36_05285) 1103474..1104157 + 684 WP_023735998.1 M15 family metallopeptidase -
NLY36_RS05310 (NLY36_05290) 1104388..1105416 + 1029 WP_023673082.1 sulfate ABC transporter substrate-binding protein -
NLY36_RS05315 (NLY36_05295) 1105435..1106286 + 852 WP_023735999.1 sulfate ABC transporter permease subunit CysT -
NLY36_RS05320 (NLY36_05300) 1106279..1107163 + 885 WP_023690809.1 sulfate ABC transporter permease subunit CysW -
NLY36_RS05325 (NLY36_05305) 1107237..1108277 + 1041 WP_023696187.1 sulfate/molybdate ABC transporter ATP-binding protein -
NLY36_RS05330 (NLY36_05310) 1108285..1108683 - 399 WP_023696188.1 type II toxin-antitoxin system VapC family toxin Toxin
NLY36_RS05335 (NLY36_05315) 1108683..1108955 - 273 WP_023687970.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
NLY36_RS05340 (NLY36_05320) 1109197..1110183 + 987 WP_023736000.1 sulfate ABC transporter substrate-binding protein -
NLY36_RS05345 (NLY36_05325) 1110443..1110892 + 450 WP_023673075.1 Rrf2 family transcriptional regulator -
NLY36_RS05350 (NLY36_05330) 1110958..1112526 - 1569 WP_023696191.1 acyl--CoA ligase -
NLY36_RS05355 (NLY36_05335) 1112613..1113758 + 1146 WP_023696192.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

SpoVT-AbrB domain

(17-55)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 13999.23 Da        Isoelectric Point: 5.7200

>T250567 WP_023696188.1 NZ_CP100472:c1108683-1108285 [Mesorhizobium sp. C399B]
MRFMLDTNILSDMIRNPAGKAASTAVCAGDGALCTSIVVASELRYGCARKGSAKLLAKVEQLLAELPVLPLDVPVDAEYG
ALRAELEARSQVIGHNDLFIAAHACALGTTLVTANTGEFSRIKGLKVENWLE

Download         Length: 399 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10187.71 Da        Isoelectric Point: 5.6343

>AT250567 WP_023687970.1 NZ_CP100472:c1108955-1108683 [Mesorhizobium sp. C399B]
MPQHRHSIPPPREAKLFRNNRSQAVRIPVEFELPGEKVLISRDGDRLIIEPVRKPGLVALLAQWAKEPPLGPEDDFPEIH
DAPVGPEDIF

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB X6J8K0

References