250562

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1316398..1317314 Replicon chromosome
Accession NZ_CP100436
Organism Bacillus subtilis strain MEC_B298

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag NLK52_RS06925 Protein ID WP_003244695.1
Coordinates 1316568..1317314 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag NLK52_RS06920 Protein ID WP_003232646.1
Coordinates 1316398..1316568 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLK52_RS06885 (NLK52_06885) 1313257..1313586 + 330 WP_015483132.1 XkdW family protein -
NLK52_RS06890 (NLK52_06890) 1313583..1313747 + 165 WP_014479563.1 XkdX family protein -
NLK52_RS06895 (NLK52_06895) 1313794..1314633 + 840 WP_015383447.1 phage-like element PBSX protein XepA -
NLK52_RS06900 (NLK52_06900) 1314686..1314955 + 270 WP_015252265.1 hemolysin XhlA family protein -
NLK52_RS06905 (NLK52_06905) 1314968..1315231 + 264 WP_015483133.1 phage holin -
NLK52_RS06910 (NLK52_06910) 1315244..1316137 + 894 WP_015483134.1 N-acetylmuramoyl-L-alanine amidase -
NLK52_RS06915 (NLK52_06915) 1316175..1316312 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
NLK52_RS06920 (NLK52_06920) 1316398..1316568 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
NLK52_RS06925 (NLK52_06925) 1316568..1317314 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
NLK52_RS06930 (NLK52_06930) 1317424..1318425 - 1002 WP_003232642.1 inorganic phosphate transporter -
NLK52_RS06935 (NLK52_06935) 1318438..1319055 - 618 WP_003218470.1 DUF47 domain-containing protein -
NLK52_RS06940 (NLK52_06940) 1319331..1320647 - 1317 WP_015383450.1 serine/threonine exchanger -
NLK52_RS06945 (NLK52_06945) 1321036..1321986 + 951 WP_015383451.1 ring-cleaving dioxygenase -
NLK52_RS06950 (NLK52_06950) 1322088..1322195 + 108 Protein_1305 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1282380..1325714 43334
- inside Prophage - - 1282380..1321986 39606


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T250562 WP_003244695.1 NZ_CP100436:c1317314-1316568 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT250562 WP_003232646.1 NZ_CP100436:c1316568-1316398 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References