Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509837..510473 Replicon chromosome
Accession NZ_CP100436
Organism Bacillus subtilis strain MEC_B298

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NLK52_RS02590 Protein ID WP_003156187.1
Coordinates 510123..510473 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NLK52_RS02585 Protein ID WP_003225183.1
Coordinates 509837..510118 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLK52_RS02565 (NLK52_02565) 506196..506795 - 600 WP_015482831.1 rhomboid family intramembrane serine protease -
NLK52_RS02570 (NLK52_02570) 506890..507255 + 366 WP_015252768.1 holo-ACP synthase -
NLK52_RS02575 (NLK52_02575) 507421..508437 + 1017 WP_080030712.1 outer membrane lipoprotein carrier protein LolA -
NLK52_RS02580 (NLK52_02580) 508552..509721 + 1170 WP_015382833.1 alanine racemase -
NLK52_RS02585 (NLK52_02585) 509837..510118 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NLK52_RS02590 (NLK52_02590) 510123..510473 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NLK52_RS02595 (NLK52_02595) 510589..511413 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NLK52_RS02600 (NLK52_02600) 511418..511783 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NLK52_RS02605 (NLK52_02605) 511787..512188 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
NLK52_RS02610 (NLK52_02610) 512200..513207 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
NLK52_RS02615 (NLK52_02615) 513269..513598 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NLK52_RS02620 (NLK52_02620) 513595..514077 + 483 WP_003234299.1 anti-sigma B factor RsbW -
NLK52_RS02625 (NLK52_02625) 514043..514831 + 789 WP_015482833.1 RNA polymerase sigma factor SigB -
NLK52_RS02630 (NLK52_02630) 514831..515430 + 600 WP_015382836.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T250561 WP_003156187.1 NZ_CP100436:510123-510473 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT250561 WP_003225183.1 NZ_CP100436:509837-510118 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References