Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1662229..1662827 Replicon chromosome
Accession NC_014915
Organism Geobacillus sp. Y412MC52

Toxin (Protein)


Gene name mazF Uniprot ID A0A0E0TBF0
Locus tag GYMC52_RS08225 Protein ID WP_013523721.1
Coordinates 1662498..1662827 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G8N5V3
Locus tag GYMC52_RS08220 Protein ID WP_011231142.1
Coordinates 1662229..1662498 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GYMC52_RS08175 1657242..1657541 + 300 WP_013523718.1 transporter suffix domain-containing protein -
GYMC52_RS08180 1657551..1658348 + 798 WP_013523719.1 SGNH/GDSL hydrolase family protein -
GYMC52_RS08185 1658345..1659259 + 915 WP_013523720.1 ABC transporter ATP-binding protein -
GYMC52_RS08190 1659252..1660232 + 981 WP_013145379.1 ABC transporter permease -
GYMC52_RS08195 1660301..1660768 - 468 WP_021321939.1 hypothetical protein -
GYMC52_RS08200 1660782..1661546 - 765 WP_011231140.1 hypothetical protein -
GYMC52_RS08220 1662229..1662498 + 270 WP_011231142.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GYMC52_RS08225 1662498..1662827 + 330 WP_013523721.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GYMC52_RS08230 1663088..1663474 + 387 WP_041469452.1 hypothetical protein -
GYMC52_RS08235 1663444..1664277 + 834 WP_013523722.1 pirin family protein -
GYMC52_RS08240 1664373..1665122 + 750 WP_013523723.1 oxygen-insensitive NADPH nitroreductase -
GYMC52_RS08245 1665312..1666055 + 744 WP_013523724.1 NAD-dependent protein deacylase -
GYMC52_RS08250 1666234..1667412 + 1179 WP_013523725.1 acyl-CoA dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-107)

Antitoxin

Antitoxin PemI-like

(12-87)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12041.83 Da        Isoelectric Point: 7.1331

>T25045 WP_013523721.1 NC_014915:1662498-1662827 [Geobacillus sp. Y412MC52]
MQAPDRGDLVYVNFNPQAGHEQAGKRPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVELPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHAFLS

Download         Length: 330 bp

>T25045 NC_014915:1662498-1662827 [Geobacillus sp. Y412MC52]
ATGCAAGCGCCGGATCGTGGGGATCTTGTTTACGTCAATTTCAATCCACAAGCAGGGCATGAGCAGGCAGGAAAAAGGCC
CGGCATCGTTCTATCACCAAAGCGGTTCAATCAATTGACAGGGTTTGCGGTGCTTTGCCCGATCACCCGGCAACAAAAAG
GATATCCATTTGAAGTGGAATTGCCATCAGGCTTGGCTGTTGAAGGCGTGATTTTAACCGATCAGGTCAAAAGTTTAGAT
TGGCGCGCCCGACAGCTTCAAATAGTAGGACGGGCGCCCAATGAAGTTGTTTCGGATTGCTTGGATCTGATTCATGCTTT
TCTTTCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9992.67 Da        Isoelectric Point: 7.2050

>AT25045 WP_011231142.1 NC_014915:1662229-1662498 [Geobacillus sp. Y412MC52]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEMIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Download         Length: 270 bp

>AT25045 NC_014915:1662229-1662498 [Geobacillus sp. Y412MC52]
ATGGAATTAAACAAACAGAAAGGAGTGTCAATCATGACAATAACAGTTCAAAAATGGGGAAACAGCCTTGCGGTTCGTAT
CCCAAGCGTGATTGCTGAACGTTTAGCGCTTCATCAAGGATCAGAAGTGGAGATGATCGTTGAGAACCAAGCGATCAAGT
TGATCCCGAAAAAGAAAAAGCCGACATTGGAGGAACTTTTGGCTAAAATCACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0TBF0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9HWH2

References