Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5187029..5187654 Replicon chromosome
Accession NZ_CP100312
Organism Klebsiella pneumoniae strain 1020

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag NKX97_RS25265 Protein ID WP_004187928.1
Coordinates 5187029..5187412 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NKX97_RS25270 Protein ID WP_004150355.1
Coordinates 5187412..5187654 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NKX97_RS25250 (5184395) 5184395..5185297 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NKX97_RS25255 (5185294) 5185294..5185929 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NKX97_RS25260 (5185926) 5185926..5186855 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NKX97_RS25265 (5187029) 5187029..5187412 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
NKX97_RS25270 (5187412) 5187412..5187654 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NKX97_RS25275 (5187848) 5187848..5188765 + 918 WP_004187929.1 alpha/beta hydrolase -
NKX97_RS25280 (5188779) 5188779..5189720 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NKX97_RS25285 (5189765) 5189765..5190202 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NKX97_RS25290 (5190199) 5190199..5191059 - 861 WP_004146232.1 virulence factor BrkB family protein -
NKX97_RS25295 (5191053) 5191053..5191652 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T250436 WP_004187928.1 NZ_CP100312:c5187412-5187029 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250436 WP_004150355.1 NZ_CP100312:c5187654-5187412 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References