Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4572549..4573359 Replicon chromosome
Accession NZ_CP100312
Organism Klebsiella pneumoniae strain 1020

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag NKX97_RS22360 Protein ID WP_023279404.1
Coordinates 4572549..4573082 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NKX97_RS22365 Protein ID WP_002887278.1
Coordinates 4573093..4573359 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NKX97_RS22355 (4571380) 4571380..4572501 + 1122 WP_002887282.1 cupin domain-containing protein -
NKX97_RS22360 (4572549) 4572549..4573082 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
NKX97_RS22365 (4573093) 4573093..4573359 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NKX97_RS22370 (4573462) 4573462..4574895 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
NKX97_RS22375 (4574885) 4574885..4575568 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NKX97_RS22380 (4575740) 4575740..4577125 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
NKX97_RS22385 (4577143) 4577143..4577487 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T250432 WP_023279404.1 NZ_CP100312:c4573082-4572549 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT250432 WP_002887278.1 NZ_CP100312:c4573359-4573093 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References