Detailed information of TA system TA025043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 44017..44551
• Replicon: plasmid pALIDE02
• Accession: NC_014911
• Organism: Alicycliphilus denitrificans BC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A4D4JP15
• Locus tag: ALIDE_RS22750
• Protein ID: WP_013521176.1
• Coordinates: 44017..44298 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4D4JMS0
• Locus tag: ALIDE_RS22755
• Protein ID: WP_013521177.1
• Coordinates: 44285..44551 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALIDE_RS22720	39142..39846	-	705	WP_013521170.1	glutathione S-transferase N-terminal domain-containing protein	-
ALIDE_RS22725	39843..40457	-	615	WP_013521171.1	TetR/AcrR family transcriptional regulator	-
ALIDE_RS22730	40521..41315	-	795	WP_013521172.1	SDR family NAD(P)-dependent oxidoreductase	-
ALIDE_RS22735	41317..42033	-	717	WP_013521173.1	glutathione S-transferase N-terminal domain-containing protein	-
ALIDE_RS22740	42030..43019	-	990	WP_013521174.1	DMT family transporter	-
ALIDE_RS22745	43016..43678	-	663	WP_013521175.1	AhpC/TSA family protein	-
ALIDE_RS22750	44017..44298	-	282	WP_013521176.1	type II toxin-antitoxin system YafQ family toxin	Toxin
ALIDE_RS22755	44285..44551	-	267	WP_013521177.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ALIDE_RS22760	45090..46310	-	1221	WP_011114072.1	replication initiator protein A	-
ALIDE_RS22765	46357..46698	-	342	WP_000019167.1	single-stranded DNA-binding protein	-
ALIDE_RS22770	46812..47174	+	363	WP_011137961.1	transcriptional regulator	-
ALIDE_RS22775	47484..48446	+	963	WP_011137962.1	P-type conjugative transfer ATPase TrbB	-
ALIDE_RS22780	48463..48927	+	465	WP_011114046.1	conjugal transfer system pilin TrbC	-
ALIDE_RS22785	48931..49242	+	312	WP_001207961.1	conjugal transfer protein TrbD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..78982	78982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10611.02 Da
• Isoelectric Point: 8.9597

>T25043 WP_013521176.1 NC_014911:c44298-44017 [Alicycliphilus denitrificans BC]
MRTIDRASAFKRDYKREAKGQHRATLDSDLKPVLVALATDQPLDAKFRDHDLSGDWAGYRECHVKPDLLLIYRKSDADTL
RLARLGSHSELFG

Nucleotide

• Length: 282 bp

>T25043 NC_014911:c44298-44017 [Alicycliphilus denitrificans BC]
ATGCGGACGATTGATCGCGCCTCAGCCTTCAAACGCGACTACAAGCGCGAGGCCAAGGGCCAGCATCGGGCTACGCTCGA
TAGCGATTTGAAGCCCGTTCTCGTGGCCCTGGCGACCGATCAGCCGCTTGACGCAAAATTCCGCGATCACGACCTGAGCG
GCGACTGGGCAGGTTATCGGGAATGCCACGTCAAGCCCGACCTGCTGCTGATCTACCGCAAGTCGGATGCCGACACTCTC
CGACTGGCACGGCTTGGCTCGCACAGCGAGCTTTTCGGTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9654.00 Da
• Isoelectric Point: 5.1392

>AT25043 WP_013521177.1 NC_014911:c44551-44285 [Alicycliphilus denitrificans BC]
MSTADTYVRARIDTSTKERAADALEAMGLSISDAIRLLMLRIADERRMPFDVKAPNATTRKAIAELEAGKGKRFASVDDL
MADLHADD

Nucleotide

• Length: 267 bp

>AT25043 NC_014911:c44551-44285 [Alicycliphilus denitrificans BC]
ATGAGCACCGCCGATACCTATGTCCGCGCTCGCATCGACACCAGCACCAAGGAGCGTGCCGCTGATGCGCTCGAAGCAAT
GGGGTTGTCCATCTCTGACGCCATCCGCTTGCTGATGCTGCGCATTGCTGATGAGCGCCGGATGCCCTTCGATGTGAAAG
CCCCTAACGCCACCACGCGCAAGGCCATTGCCGAACTTGAGGCTGGGAAAGGCAAGCGTTTCGCCAGCGTTGATGACCTG
ATGGCAGACTTGCATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A4D4JP15

Antitoxin

• AlphaFold DB: A0A4D4JMS0