Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 16575..17311 Replicon plasmid pKPN_1021
Accession NZ_CP100310
Organism Klebsiella pneumoniae strain 1021

Toxin (Protein)


Gene name tacT Uniprot ID A0A486JQ35
Locus tag NKY02_RS26380 Protein ID WP_049109282.1
Coordinates 16829..17311 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag NKY02_RS26375 Protein ID WP_003026799.1
Coordinates 16575..16841 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NKY02_RS26355 (NKY02_26340) 12655..14211 - 1557 Protein_10 DUF4158 domain-containing protein -
NKY02_RS26360 (NKY02_26345) 14265..14969 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
NKY02_RS26365 (NKY02_26350) 15324..16139 + 816 WP_049109278.1 hypothetical protein -
NKY02_RS26370 (NKY02_26355) 16182..16289 - 108 Protein_13 transposase -
NKY02_RS26375 (NKY02_26360) 16575..16841 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
NKY02_RS26380 (NKY02_26365) 16829..17311 + 483 WP_049109282.1 GNAT family N-acetyltransferase Toxin
NKY02_RS26385 (NKY02_26370) 17511..18914 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
NKY02_RS26390 (NKY02_26375) 18943..19575 - 633 WP_001567369.1 hypothetical protein -
NKY02_RS26395 (NKY02_26380) 19752..21287 - 1536 Protein_18 IS3 family transposase -
NKY02_RS26400 (NKY02_26385) 21431..21838 + 408 WP_023304028.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..112694 112694
- inside IScluster/Tn - - 12604..20789 8185


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17336.86 Da        Isoelectric Point: 8.0020

>T250421 WP_049109282.1 NZ_CP100310:16829-17311 [Klebsiella pneumoniae]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCETGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT250421 WP_003026799.1 NZ_CP100310:16575-16841 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486JQ35

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References