Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4573742..4574552 Replicon chromosome
Accession NZ_CP100308
Organism Klebsiella pneumoniae strain 1021

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag NKY02_RS22370 Protein ID WP_023279404.1
Coordinates 4573742..4574275 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NKY02_RS22375 Protein ID WP_002887278.1
Coordinates 4574286..4574552 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NKY02_RS22365 (4572573) 4572573..4573694 + 1122 WP_002887282.1 cupin domain-containing protein -
NKY02_RS22370 (4573742) 4573742..4574275 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
NKY02_RS22375 (4574286) 4574286..4574552 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NKY02_RS22380 (4574655) 4574655..4576088 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
NKY02_RS22385 (4576078) 4576078..4576761 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NKY02_RS22390 (4576933) 4576933..4578318 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
NKY02_RS22395 (4578336) 4578336..4578680 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T250415 WP_023279404.1 NZ_CP100308:c4574275-4573742 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT250415 WP_002887278.1 NZ_CP100308:c4574552-4574286 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References