Detailed information of TA system TA025041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1941276..1942033
• Replicon: chromosome
• Accession: NC_014910
• Organism: Alicycliphilus denitrificans BC

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: ALIDE_RS23445
• Protein ID: WP_013518725.1
• Coordinates: 1941276..1941770 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: F4G8U8
• Locus tag: ALIDE_RS09335
• Protein ID: WP_013518726.1
• Coordinates: 1941767..1942033 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALIDE_RS09310	1936681..1937868	-	1188	WP_013518721.1	amidohydrolase	-
ALIDE_RS09315	1937906..1939225	-	1320	WP_180987920.1	MFS transporter	-
ALIDE_RS09320	1939373..1940275	+	903	WP_013518723.1	LysR family transcriptional regulator	-
ALIDE_RS24125	1940405..1940656	-	252	Protein_1870	IS1595 family transposase	-
ALIDE_RS23445	1941276..1941770	-	495	WP_013518725.1	GNAT family N-acetyltransferase	Toxin
ALIDE_RS09335	1941767..1942033	-	267	WP_013518726.1	DUF1778 domain-containing protein	Antitoxin
ALIDE_RS09340	1942264..1943520	-	1257	WP_013518727.1	MFS transporter	-
ALIDE_RS09345	1943704..1944495	+	792	WP_013518728.1	SDR family oxidoreductase	-
ALIDE_RS09350	1944559..1944984	+	426	WP_013518729.1	PaaI family thioesterase	-
ALIDE_RS09355	1945078..1946094	+	1017	WP_013518730.1	NADP-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17883.59 Da
• Isoelectric Point: 8.4881

>T25041 WP_013518725.1 NC_014910:c1941770-1941276 [Alicycliphilus denitrificans BC]
MSLDLVAPQPLTSDHHCIDFSCGEPVLDEWLKRRAMANQISGASRTFVAADKEHRVYGYYAMAAGAVSHQLATGSVRRNM
PDPVPVMVLARLAVDTQAQGIKLGGSLLQDAVNRAVNVAQNTGVRALLVHALHERAKLFYEYYGFQASPADPLVLMLRLS
QAKP

Nucleotide

• Length: 495 bp

>T25041 NC_014910:c1941770-1941276 [Alicycliphilus denitrificans BC]
ATGAGCCTTGATCTTGTCGCGCCGCAGCCGCTGACGTCAGATCATCACTGCATCGATTTTTCCTGCGGCGAACCGGTGCT
GGATGAATGGCTCAAGCGTCGGGCGATGGCCAATCAGATCAGCGGAGCCAGTCGCACTTTTGTTGCGGCGGATAAAGAGC
ACCGTGTTTATGGCTACTACGCAATGGCGGCTGGTGCTGTGTCCCATCAGCTCGCCACAGGCAGTGTGCGCAGAAACATG
CCTGATCCGGTGCCCGTCATGGTATTGGCCAGGCTTGCCGTCGACACCCAGGCCCAGGGCATCAAGCTGGGTGGCTCCCT
ACTGCAGGATGCCGTCAACCGGGCGGTGAACGTGGCGCAGAACACGGGCGTTCGTGCCTTGCTGGTTCATGCCCTGCACG
AGCGTGCGAAGTTGTTTTACGAGTATTACGGCTTCCAAGCATCTCCGGCTGATCCGCTGGTACTGATGCTGCGCCTGAGC
CAGGCCAAGCCGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9927.40 Da
• Isoelectric Point: 5.6458

>AT25041 WP_013518726.1 NC_014910:c1942033-1941767 [Alicycliphilus denitrificans BC]
MRAAAINLRALPEQRDLIDHAANLLGKNRSDFMLEAACERAQSVVLDQVHFTLDADKFQQFVDLLDAPPQPNTGLERLFA
LKPVWNKV

Nucleotide

• Length: 267 bp

>AT25041 NC_014910:c1942033-1941767 [Alicycliphilus denitrificans BC]
ATGCGTGCTGCCGCCATCAATCTGCGTGCTCTGCCCGAGCAGCGTGACCTCATCGATCACGCCGCCAACCTGCTGGGAAA
GAACCGTTCGGACTTTATGCTGGAGGCCGCCTGCGAGCGTGCCCAGTCTGTTGTGCTTGATCAGGTTCACTTCACGCTGG
ATGCTGACAAATTCCAGCAGTTTGTCGATCTACTCGATGCGCCACCCCAGCCCAACACCGGGCTGGAACGCCTGTTTGCA
CTGAAGCCTGTCTGGAACAAGGTATGA

Structures

Antitoxin

• AlphaFold DB: F4G8U8