Detailed information of TA system TA025040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1769104..1769706
• Replicon: chromosome
• Accession: NC_014910
• Organism: Alicycliphilus denitrificans BC

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ALIDE_RS08475
• Protein ID: WP_013518571.1
• Coordinates: 1769524..1769706 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ALIDE_RS08470
• Protein ID: WP_013518570.1
• Coordinates: 1769104..1769496 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALIDE_RS08430	1764649..1765068	+	420	WP_013518562.1	hypothetical protein	-
ALIDE_RS08435	1765065..1765601	+	537	WP_013518563.1	hypothetical protein	-
ALIDE_RS08440	1765605..1766123	+	519	WP_013518564.1	hypothetical protein	-
ALIDE_RS08445	1766135..1767376	+	1242	WP_013518565.1	hypothetical protein	-
ALIDE_RS08450	1767437..1767742	+	306	WP_013518566.1	hypothetical protein	-
ALIDE_RS08455	1767739..1768323	+	585	WP_013518567.1	lysozyme	-
ALIDE_RS08460	1768320..1768583	+	264	WP_013518568.1	hypothetical protein	-
ALIDE_RS08465	1768624..1769091	+	468	WP_013518569.1	hypothetical protein	-
ALIDE_RS08470	1769104..1769496	-	393	WP_013518570.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ALIDE_RS08475	1769524..1769706	-	183	WP_013518571.1	type II toxin-antitoxin system HicA family toxin	Toxin
ALIDE_RS08480	1769798..1770088	+	291	WP_013518572.1	hypothetical protein	-
ALIDE_RS08485	1770085..1770345	+	261	WP_013518573.1	hypothetical protein	-
ALIDE_RS08490	1770593..1770790	+	198	WP_148237975.1	hypothetical protein	-
ALIDE_RS24075	1771237..1771473	-	237	WP_013518574.1	hypothetical protein	-
ALIDE_RS08495	1771476..1771712	-	237	WP_013518575.1	hypothetical protein	-
ALIDE_RS08500	1771805..1772173	-	369	WP_041715911.1	tyrosine-type recombinase/integrase	-
ALIDE_RS24080	1772450..1772701	-	252	WP_135149032.1	hypothetical protein	-
ALIDE_RS24085	1773128..1773415	-	288	WP_148238012.1	tyrosine-type recombinase/integrase	-
ALIDE_RS24560	1773395..1773750	-	356	Protein_1702	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1711662..1799946	88284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-122)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6627.90 Da
• Isoelectric Point: 11.8943

>T25040 WP_013518571.1 NC_014910:c1769706-1769524 [Alicycliphilus denitrificans BC]
MNSAALIKQIKADGWVLVRTVGSHHQFKHPTKPGKVTVPHPKKDLPLPTVRSILKQAGLR

Nucleotide

• Length: 183 bp

>T25040 NC_014910:c1769706-1769524 [Alicycliphilus denitrificans BC]
ATGAACAGCGCAGCACTCATCAAGCAGATCAAGGCAGACGGTTGGGTGCTGGTGCGCACGGTCGGCTCTCACCATCAGTT
CAAGCACCCGACCAAGCCGGGCAAGGTCACAGTACCTCACCCGAAGAAAGACCTGCCGCTGCCCACTGTTCGCAGCATCC
TCAAGCAAGCAGGGCTCCGCTAA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 13879.54 Da
• Isoelectric Point: 4.3280

>AT25040 WP_013518570.1 NC_014910:c1769496-1769104 [Alicycliphilus denitrificans BC]
MLYPIYVHKDADSAYGAIFPDFPGCFAAADDLQGLPRAAQEAAEAHFGADDDPIPAPTSPEAWAADPDYSGGFWMLVEID
LSKVRAKAVRLNISLNESLLQRIDLAAKARGQSRSAFLATAAEHEMTGAA

Nucleotide

• Length: 393 bp

>AT25040 NC_014910:c1769496-1769104 [Alicycliphilus denitrificans BC]
ATGCTCTATCCGATCTACGTACACAAGGACGCCGATAGCGCCTACGGTGCGATCTTCCCGGACTTCCCCGGCTGCTTCGC
TGCGGCCGATGATCTGCAGGGCCTGCCCCGTGCTGCCCAGGAAGCCGCGGAGGCGCACTTCGGCGCGGATGATGATCCGA
TCCCCGCCCCTACCTCTCCCGAAGCATGGGCGGCCGATCCAGACTACTCGGGCGGGTTCTGGATGCTGGTGGAGATCGAC
TTGTCCAAGGTGCGGGCCAAGGCCGTGCGGCTGAACATCAGCCTGAACGAGAGTCTGTTGCAGCGGATCGACCTCGCCGC
GAAGGCGCGCGGGCAGTCGCGCTCGGCGTTTCTCGCCACCGCGGCAGAGCACGAGATGACTGGGGCGGCGTAG