Detailed information of TA system TA025039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 102214..102755
• Replicon: plasmid pALIDE01
• Accession: NC_014908
• Organism: Alicycliphilus denitrificans BC

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ALIDE_RS22470
• Protein ID: WP_013516327.1
• Coordinates: 102214..102537 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ALIDE_RS23800
• Protein ID: WP_013516328.1
• Coordinates: 102534..102755 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALIDE_RS22460	97530..100262	-	2733	WP_148238052.1	DEAD/DEAH box helicase family protein	-
ALIDE_RS22465	100286..102214	-	1929	WP_013516326.1	site-specific DNA-methyltransferase	-
ALIDE_RS22470	102214..102537	-	324	WP_013516327.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ALIDE_RS23800	102534..102755	-	222	WP_013516328.1	antitoxin MazE family protein	Antitoxin
ALIDE_RS22475	102831..103667	+	837	WP_013516329.1	restriction endonuclease	-
ALIDE_RS22480	103685..104056	-	372	WP_013516330.1	HU family DNA-binding protein	-
ALIDE_RS22485	104214..104666	+	453	WP_013516331.1	helix-turn-helix domain-containing protein	-
ALIDE_RS22490	104677..105234	-	558	WP_013516332.1	hypothetical protein	-
ALIDE_RS22495	105502..107238	+	1737	Protein_122	Tn3-like element IS1071 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..119718	119718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (9-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11729.70 Da
• Isoelectric Point: 6.2970

>T25039 WP_013516327.1 NC_014908:c102537-102214 [Alicycliphilus denitrificans BC]
MTQRGDIVAVSIQGDYGKPRPALVIQSDLLEGLQSVVLCPITSELRDATFRVTVEPNPANGLRVLSQVMADKISTLPRNK
LSEPFGRLDDDRMKAVERALLLVIGMI

Nucleotide

• Length: 324 bp

>T25039 NC_014908:c102537-102214 [Alicycliphilus denitrificans BC]
ATGACCCAACGCGGCGACATTGTTGCTGTTTCCATCCAAGGGGACTACGGCAAACCGCGCCCAGCTCTGGTCATCCAGTC
CGATCTGCTCGAAGGCTTGCAAAGCGTCGTGCTCTGCCCGATCACCAGCGAGCTACGTGACGCAACGTTTCGCGTGACTG
TTGAGCCGAACCCGGCCAATGGGCTGCGTGTGCTTTCACAGGTGATGGCCGACAAGATTTCGACCCTGCCACGCAACAAG
CTCAGCGAGCCCTTCGGCCGTCTCGATGACGATCGCATGAAGGCCGTTGAGCGCGCGCTCCTACTCGTGATTGGAATGAT
TTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8491.56 Da
• Isoelectric Point: 5.1104

>AT25039 WP_013516328.1 NC_014908:c102755-102534 [Alicycliphilus denitrificans BC]
MATSASSIDKVARYRERMRASGLRLVQFWVPDTRSAEFMARVREQCQRLAEDPQEEQALRFTQSAAELIEGWE

Nucleotide

• Length: 222 bp

>AT25039 NC_014908:c102755-102534 [Alicycliphilus denitrificans BC]
ATGGCGACCTCCGCATCTTCGATAGACAAGGTAGCGCGCTACCGTGAGCGCATGCGCGCCTCAGGCCTGCGGCTAGTGCA
GTTCTGGGTCCCTGACACCCGTTCGGCCGAATTCATGGCACGTGTACGCGAGCAATGCCAGCGCTTAGCCGAGGATCCTC
AGGAAGAACAAGCCTTGAGGTTTACGCAATCAGCCGCTGAGCTCATAGAGGGCTGGGAATGA