Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 128913..129508 Replicon plasmid p48s2p
Accession NZ_CP100288
Organism Burkholderia glumae strain BGR48S

Toxin (Protein)


Gene name chpB Uniprot ID C5AN78
Locus tag NIX85_RS29730 Protein ID WP_012734107.1
Coordinates 128913..129263 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID C5AN79
Locus tag NIX85_RS29735 Protein ID WP_012734108.1
Coordinates 129257..129508 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIX85_RS29705 124135..124881 - 747 WP_232252306.1 energy transducer TonB -
NIX85_RS29710 124913..127300 - 2388 WP_257820390.1 TonB-dependent receptor -
NIX85_RS29715 127602..128066 - 465 WP_012734106.1 Lrp/AsnC family transcriptional regulator -
NIX85_RS29720 128289..128510 - 222 WP_017433768.1 hypothetical protein -
NIX85_RS29725 128660..128854 - 195 WP_124837685.1 hypothetical protein -
NIX85_RS29730 128913..129263 - 351 WP_012734107.1 type II toxin-antitoxin system ChpB family toxin Toxin
NIX85_RS29735 129257..129508 - 252 WP_012734108.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
NIX85_RS29740 129771..130178 - 408 WP_230674618.1 hypothetical protein -
NIX85_RS29745 130281..132116 - 1836 WP_012732711.1 site-specific integrase -
NIX85_RS29750 132119..132460 - 342 WP_012732710.1 hypothetical protein -
NIX85_RS29755 132517..132762 - 246 WP_127913916.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..132942 132942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

Antitoxin PemI-like

(1-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12389.37 Da        Isoelectric Point: 8.8909

>T250380 WP_012734107.1 NZ_CP100288:c129263-128913 [Burkholderia glumae]
MVKRVKFERGDIVRVSLSPTVGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEGRGARKIERAPVEVVEDALARLQTIIE

Download         Length: 351 bp

>T250380 NZ_CP100288:c129263-128913 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 8940.31 Da        Isoelectric Point: 6.2420

>AT250380 WP_012734108.1 NZ_CP100288:c129508-129257 [Burkholderia glumae]
MELKIQKWGNSAAVRLPSVLLEQINASVGGSLNADVRPDGVLLTPARRKYALDDLVAQCDTKAPVPADMAAWGEIKPVGR
EAW

Download         Length: 252 bp

>AT250380 NZ_CP100288:c129508-129257 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246MF70

Antitoxin

Source ID Structure
AlphaFold DB A0A246MFN5

References