Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 36840..37417 Replicon plasmid p35s2p
Accession NZ_CP100253
Organism Burkholderia glumae strain BGR35S

Toxin (Protein)


Gene name vapC Uniprot ID F2LRT1
Locus tag NIX82_RS30345 Protein ID WP_013699949.1
Coordinates 37052..37417 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F2LRT2
Locus tag NIX82_RS30340 Protein ID WP_013699950.1
Coordinates 36840..37058 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIX82_RS30320 32249..32602 + 354 WP_012732734.1 IS66 family insertion sequence element accessory protein TnpB -
NIX82_RS30325 32702..34288 + 1587 WP_012732733.1 IS66 family transposase -
NIX82_RS30330 34592..35632 + 1041 WP_257829937.1 hypothetical protein -
NIX82_RS30335 35676..36374 - 699 WP_257829938.1 hypothetical protein -
NIX82_RS30340 36840..37058 + 219 WP_013699950.1 ribbon-helix-helix protein, CopG family Antitoxin
NIX82_RS30345 37052..37417 + 366 WP_013699949.1 type II toxin-antitoxin system VapC family toxin Toxin
NIX82_RS30350 37461..37643 + 183 WP_148270663.1 hypothetical protein -
NIX82_RS30355 37710..37955 + 246 WP_257829939.1 hypothetical protein -
NIX82_RS30360 38371..39417 + 1047 WP_257829940.1 IS481 family transposase -
NIX82_RS30365 39447..39572 - 126 WP_257829942.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..172021 172021
- inside IScluster/Tn - - 32249..39417 7168


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(4-41)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13467.63 Da        Isoelectric Point: 8.4446

>T250353 WP_013699949.1 NZ_CP100253:37052-37417 [Burkholderia glumae]
MVKALFDTNILIDYLGGIAAAKKELGRYEYRAISTITWMEVLVGASSGEEDAIRGWLDSFDVIPVGSDIANRAVVIRKAR
RIRLPDAIIWATAQVHSLLLVSRNTKDFPPDEPGVRIPYKL

Download         Length: 366 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8169.35 Da        Isoelectric Point: 5.0490

>AT250353 WP_013699950.1 NZ_CP100253:36840-37058 [Burkholderia glumae]
MSRILVDLPDSQVDDLAVIVEAEQRPRAAVIRDAIEAYIAQRKRALGADVFGLWKSKKVDGLAYQQELRSEW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F2LRT1

Antitoxin

Source ID Structure
AlphaFold DB F2LRT2

References