Detailed information of TA system TA025034

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 336363..337036
• Replicon: plasmid pPAT9B01
• Accession: NC_014838
• Organism: Pantoea sp. At-9b

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: PAT9B_RS21940
• Protein ID: WP_083810354.1
• Coordinates: 336363..336761 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: PAT9B_RS21945
• Protein ID: WP_013511473.1
• Coordinates: 336782..337036 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAT9B_RS21910	332536..332691	+	156	WP_013511466.1	type I toxin-antitoxin system Hok family toxin	-
PAT9B_RS21915	332768..333397	-	630	WP_013511467.1	DUF1109 family protein	-
PAT9B_RS21920	333390..333941	-	552	WP_013511468.1	sigma-70 family RNA polymerase sigma factor	-
PAT9B_RS21925	333958..335190	-	1233	WP_013511469.1	thioredoxin family protein	-
PAT9B_RS21930	335206..335775	-	570	WP_013511470.1	peroxiredoxin	-
PAT9B_RS21935	336042..336320	+	279	Protein_349	hypothetical protein	-
PAT9B_RS21940	336363..336761	-	399	WP_083810354.1	HipA domain-containing protein	Toxin
PAT9B_RS21945	336782..337036	-	255	WP_013511473.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
PAT9B_RS21950	337297..337752	+	456	WP_013511474.1	acetyl-CoA carboxylase biotin carboxyl carrier protein	-
PAT9B_RS21955	337764..339113	+	1350	WP_013511475.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
PAT9B_RS21960	339129..340400	+	1272	WP_013511476.1	Glu/Leu/Phe/Val dehydrogenase	-
PAT9B_RS21965	340510..341589	-	1080	WP_013511477.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..793953	793953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (3-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14988.53 Da
• Isoelectric Point: 10.3341

>T25034 WP_083810354.1 NC_014838:c336761-336363 [Pantoea sp. At-9b]
MNGATDGHAKNFSVFIERGGSYRLTPFYDVISAYPVLGGTGLNIRDLKLAMGLKATKGKKMEIEKIFPRHFMATAKAVNF
DQNRMVEIMDFFITQFPDAIRQVERMLPADFPPRIADVLFSQSLRMLARLQQ

Nucleotide

• Length: 399 bp

>T25034 NC_014838:c336761-336363 [Pantoea sp. At-9b]
ATGAATGGTGCCACCGACGGACATGCGAAAAACTTCTCGGTATTTATTGAGCGAGGGGGCAGCTATCGCCTGACACCTTT
CTATGATGTCATTTCGGCTTACCCCGTGTTGGGTGGAACCGGATTGAACATCCGTGATCTGAAACTGGCGATGGGGTTAA
AAGCCACGAAAGGTAAAAAGATGGAGATTGAGAAAATATTCCCACGGCATTTTATGGCAACGGCTAAGGCAGTAAATTTT
GACCAGAACCGCATGGTGGAAATTATGGATTTCTTTATTACGCAATTCCCAGACGCCATCCGCCAAGTAGAACGTATGTT
ACCCGCTGATTTCCCGCCCCGTATCGCCGATGTACTGTTTTCCCAAAGCCTGCGAATGCTGGCGCGTTTACAGCAATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9840.20 Da
• Isoelectric Point: 7.4013

>AT25034 WP_013511473.1 NC_014838:c337036-336782 [Pantoea sp. At-9b]
MIYSPRQLAHHIRLIRQKNQWTQTQLAKKVGLKQATISHFENNPDLTTLATLFKILQSLELKLDIHEKLDANETPASYDQ
ENDW

Nucleotide

• Length: 255 bp

>AT25034 NC_014838:c337036-336782 [Pantoea sp. At-9b]
ATGATTTATAGCCCAAGACAGCTTGCTCATCACATCCGGTTAATTCGACAGAAAAATCAGTGGACCCAGACGCAACTCGC
CAAAAAAGTGGGGCTGAAACAGGCCACGATCTCACATTTCGAAAACAATCCCGACCTGACCACCCTGGCGACGCTGTTTA
AGATCCTGCAGTCCCTTGAACTAAAGCTGGATATTCACGAGAAGCTGGACGCAAACGAGACTCCGGCGAGTTACGATCAG
GAGAATGACTGGTGA