Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1694027..1694802 Replicon chromosome
Accession NZ_CP100228
Organism Burkholderia glumae strain SEW1RS

Toxin (Protein)


Gene name TacT2 Uniprot ID C5AIF4
Locus tag NIX03_RS25420 Protein ID WP_015877380.1
Coordinates 1694027..1694524 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID C5AIF5
Locus tag NIX03_RS25425 Protein ID WP_015877381.1
Coordinates 1694521..1694802 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIX03_RS25385 1690048..1690143 - 96 Protein_1326 IS5/IS1182 family transposase -
NIX03_RS25390 1690215..1690760 - 546 Protein_1327 IS3 family transposase -
NIX03_RS25395 1690953..1691758 + 806 WP_085962370.1 IS5 family transposase -
NIX03_RS25400 1691724..1692035 - 312 WP_015877377.1 hypothetical protein -
NIX03_RS25405 1692052..1692354 - 303 WP_255220127.1 DUF2380 domain-containing protein -
NIX03_RS25410 1692430..1693035 - 606 Protein_1331 RHS repeat-associated core domain-containing protein -
NIX03_RS25415 1693449..1693946 - 498 WP_015877379.1 ProQ/FINO family protein -
NIX03_RS25420 1694027..1694524 - 498 WP_015877380.1 GNAT family N-acetyltransferase Toxin
NIX03_RS25425 1694521..1694802 - 282 WP_015877381.1 DUF1778 domain-containing protein Antitoxin
NIX03_RS25430 1694799..1695176 - 378 WP_173941242.1 hypothetical protein -
NIX03_RS25435 1695259..1695621 - 363 WP_017432434.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fliC 1678396..1720350 41954
- flank IS/Tn - - 1690191..1690733 542


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-144)

Antitoxin

Vibrio phage ICP1, Orf50

(5-91)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 17544.11 Da        Isoelectric Point: 9.6894

>T250335 WP_015877380.1 NZ_CP100228:c1694524-1694027 [Burkholderia glumae]
VTGWRAPEPLSDAHGLDDFDSGVASLDTWLKRRALANQRSGASRTFVATRDGRVGAYYALASGAVTLEVAPGRFRRNMPD
PIPVVVLGRLAVDRAHQGIGVGRALVRDAGLRVLHAAAAIGIRGLIVHALSDSAKAFYERVGFEASPLDPMLLLITLADL
EHALS

Download         Length: 498 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10256.52 Da        Isoelectric Point: 5.0477

>AT250335 WP_015877381.1 NZ_CP100228:c1694802-1694521 [Burkholderia glumae]
MTSTTPRDTLNLRIRPDDRSLIDRAAAAAHKTRTDFVLDAARAAAEQTLLDQVVLSAEPQAYAAFLARLDEPVQPNEALR
QTMQTRAPWDPAA

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J9HNC8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J9HNC9

References