Detailed information of TA system TA025032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PrpT-parD/ParE-YefM
• Location: 205101..205648
• Replicon: plasmid pPAT9B01
• Accession: NC_014838
• Organism: Pantoea sp. At-9b

Toxin (Protein)

• Gene name: PrpT
• Uniprot ID: E6WK78
• Locus tag: PAT9B_RS21190
• Protein ID: WP_013511319.1
• Coordinates: 205101..205388 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E6WK79
• Locus tag: PAT9B_RS21195
• Protein ID: WP_013511320.1
• Coordinates: 205385..205648 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAT9B_RS21170	200829..202364	+	1536	WP_013511315.1	glycosyl transferase	-
PAT9B_RS21175	202381..203301	+	921	WP_013511316.1	ABC transporter permease	-
PAT9B_RS21180	203301..204167	+	867	WP_013511317.1	ABC transporter permease	-
PAT9B_RS21185	204164..205090	+	927	WP_013511318.1	ROK family protein	-
PAT9B_RS21190	205101..205388	-	288	WP_013511319.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PAT9B_RS21195	205385..205648	-	264	WP_013511320.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PAT9B_RS21200	205727..206602	-	876	WP_013511321.1	LysR family transcriptional regulator	-
PAT9B_RS21205	206726..207451	+	726	WP_013511322.1	pirin family protein	-
PAT9B_RS21210	207492..208490	-	999	WP_013511323.1	inositol 2-dehydrogenase	-
PAT9B_RS21215	208727..209752	+	1026	WP_013511324.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..793953	793953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-64)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10873.65 Da
• Isoelectric Point: 9.9233

>T25032 WP_013511319.1 NC_014838:c205388-205101 [Pantoea sp. At-9b]
MTLKISQEAQEDIHDIRRYTAKNWGKAKAIEYILQLQLTMKTLEKSPQLGIERDEIRAGARSFPCGRHIIFYRALADGIV
VTAVLGQLQDPKRHL

Nucleotide

• Length: 288 bp

>T25032 NC_014838:c205388-205101 [Pantoea sp. At-9b]
ATGACACTGAAAATCTCGCAGGAAGCGCAAGAAGATATTCACGACATTCGTCGATATACCGCTAAGAACTGGGGCAAGGC
AAAAGCCATTGAATATATTTTGCAATTGCAGCTGACCATGAAGACCCTGGAGAAATCTCCGCAATTGGGTATTGAGCGCG
ATGAAATTCGTGCCGGAGCCCGCAGTTTCCCTTGCGGCCGACACATCATTTTTTACCGTGCGCTTGCCGATGGTATTGTT
GTTACTGCTGTGTTGGGGCAACTTCAGGATCCAAAGCGACATTTGTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9890.33 Da
• Isoelectric Point: 4.5482

>AT25032 WP_013511320.1 NC_014838:c205648-205385 [Pantoea sp. At-9b]
METITAQVAKTQFGELLMKAQREPVEISKHGKRVAVLMSAEEFDQIEALKLQMLRDKLARAEADIAAGRSMDGDQFFNQL
MKEFDDE

Nucleotide

• Length: 264 bp

>AT25032 NC_014838:c205648-205385 [Pantoea sp. At-9b]
ATGGAAACCATAACTGCACAGGTGGCGAAAACCCAGTTTGGCGAACTGCTGATGAAGGCACAGCGCGAACCAGTGGAGAT
AAGCAAACACGGTAAGCGTGTCGCAGTGCTGATGTCTGCTGAAGAGTTTGACCAGATCGAGGCATTAAAATTACAGATGC
TGCGTGATAAGCTGGCCCGCGCCGAAGCCGATATTGCCGCTGGTCGTTCTATGGATGGCGATCAATTCTTCAACCAACTA
ATGAAAGAGTTTGATGACGAATGA

Structures

Toxin

• AlphaFold DB: E6WK78

Antitoxin

• AlphaFold DB: E6WK79