Detailed information of TA system TA250280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1694020..1694795
• Replicon: chromosome
• Accession: NZ_CP100151
• Organism: Burkholderia glumae strain BGR82S

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: C5AIF4
• Locus tag: NIV83_RS25440
• Protein ID: WP_015877380.1
• Coordinates: 1694020..1694517 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: C5AIF5
• Locus tag: NIV83_RS25445
• Protein ID: WP_015877381.1
• Coordinates: 1694514..1694795 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NIV83_RS25405	1690041..1690136	-	96	Protein_1328	IS5/IS1182 family transposase	-
NIV83_RS25410	1690208..1690753	-	546	Protein_1329	IS3 family transposase	-
NIV83_RS25415	1690946..1691751	+	806	WP_085962370.1	IS5 family transposase	-
NIV83_RS25420	1691717..1692028	-	312	WP_015877377.1	hypothetical protein	-
NIV83_RS25425	1692045..1692347	-	303	WP_255220127.1	DUF2380 domain-containing protein	-
NIV83_RS25430	1692423..1693028	-	606	Protein_1333	RHS repeat-associated core domain-containing protein	-
NIV83_RS25435	1693442..1693939	-	498	WP_015877379.1	ProQ/FINO family protein	-
NIV83_RS25440	1694020..1694517	-	498	WP_015877380.1	GNAT family N-acetyltransferase	Toxin
NIV83_RS25445	1694514..1694795	-	282	WP_015877381.1	DUF1778 domain-containing protein	Antitoxin
NIV83_RS25450	1694792..1695169	-	378	WP_173941242.1	hypothetical protein	-
NIV83_RS25455	1695252..1695614	-	363	WP_017432434.1	hypothetical protein	-
NIV83_RS25460	1695623..1699750	-	4128	WP_275995111.1	RHS repeat-associated core domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fliC	1678027..1720343	42316
	-	flank	IS/Tn	-	-	1690184..1690726	542

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-91)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 17544.11 Da
• Isoelectric Point: 9.6894

>T250280 WP_015877380.1 NZ_CP100151:c1694517-1694020 [Burkholderia glumae]
VTGWRAPEPLSDAHGLDDFDSGVASLDTWLKRRALANQRSGASRTFVATRDGRVGAYYALASGAVTLEVAPGRFRRNMPD
PIPVVVLGRLAVDRAHQGIGVGRALVRDAGLRVLHAAAAIGIRGLIVHALSDSAKAFYERVGFEASPLDPMLLLITLADL
EHALS

Nucleotide

• Length: 498 bp

>T250280 NZ_CP100151:c1694517-1694020 [Burkholderia glumae]
GTGACGGGCTGGCGCGCGCCCGAACCGCTCAGCGACGCGCACGGGCTCGACGATTTCGATTCCGGCGTCGCGAGCCTCGA
CACGTGGCTCAAACGCCGTGCACTGGCCAATCAGCGTAGCGGTGCGTCGCGGACCTTTGTGGCGACGCGAGACGGTCGAG
TGGGGGCGTACTACGCACTCGCGTCCGGCGCGGTTACGCTCGAGGTCGCACCGGGCCGCTTCCGGCGCAACATGCCCGAT
CCGATACCGGTGGTTGTGCTCGGGCGTCTCGCCGTCGATCGCGCCCATCAGGGCATTGGGGTTGGGCGTGCGCTGGTACG
CGATGCCGGCTTGCGTGTGTTGCACGCCGCAGCGGCCATCGGGATTCGCGGCCTGATCGTGCATGCACTCTCGGACTCGG
CCAAGGCGTTCTATGAGCGGGTCGGCTTTGAGGCGTCGCCGCTCGATCCGATGCTGCTCCTGATCACGCTGGCCGACCTC
GAGCACGCCTTGTCATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10256.52 Da
• Isoelectric Point: 5.0477

>AT250280 WP_015877381.1 NZ_CP100151:c1694795-1694514 [Burkholderia glumae]
MTSTTPRDTLNLRIRPDDRSLIDRAAAAAHKTRTDFVLDAARAAAEQTLLDQVVLSAEPQAYAAFLARLDEPVQPNEALR
QTMQTRAPWDPAA

Nucleotide

• Length: 282 bp

>AT250280 NZ_CP100151:c1694795-1694514 [Burkholderia glumae]
ATGACCTCGACCACCCCACGCGACACTCTGAACCTACGCATTCGTCCTGACGATCGCAGCTTGATTGACCGCGCCGCAGC
GGCCGCCCACAAAACGCGTACCGACTTCGTTCTGGACGCAGCGCGGGCCGCCGCCGAACAGACCTTGCTCGACCAGGTTG
TTCTGAGCGCCGAGCCGCAGGCCTACGCGGCCTTCCTGGCGCGTTTGGACGAGCCCGTCCAACCGAACGAGGCATTGCGC
CAGACCATGCAGACACGCGCGCCCTGGGACCCCGCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A2J9HNC8

Antitoxin

• AlphaFold DB: A0A2J9HNC9