Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 162390..162915 Replicon plasmid pGZKP13-1
Accession NZ_CP100121
Organism Klebsiella pneumoniae strain GZKP13

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag NIK98_RS27580 Protein ID WP_001159871.1
Coordinates 162390..162695 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag NIK98_RS27585 Protein ID WP_000813634.1
Coordinates 162697..162915 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIK98_RS27545 (158144) 158144..158788 + 645 WP_000633913.1 division plane positioning ATPase MipZ -
NIK98_RS27550 (158782) 158782..159057 + 276 WP_000239529.1 hypothetical protein -
NIK98_RS27555 (159249) 159249..159675 - 427 Protein_192 site-specific integrase -
NIK98_RS27560 (159855) 159855..160445 + 591 Protein_193 colicin-B -
NIK98_RS27570 (161214) 161214..161408 - 195 Protein_195 colicin M immunity protein -
NIK98_RS27575 (161580) 161580..162389 - 810 WP_000016963.1 site-specific integrase -
NIK98_RS27580 (162390) 162390..162695 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
NIK98_RS27585 (162697) 162697..162915 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
NIK98_RS27590 (163476) 163476..163706 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
NIK98_RS27595 (163703) 163703..164119 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
NIK98_RS27600 (164194) 164194..165759 + 1566 WP_001128474.1 AAA family ATPase -
NIK98_RS27605 (165744) 165744..166766 + 1023 WP_000361402.1 helicase UvrD -
NIK98_RS27610 (167020) 167020..167706 - 687 Protein_203 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aph(6)-Id / aph(3'')-Ib / catA1 / aph(3')-Ia / mph(A) / dfrA12 / aadA2 / qacE / sul1 / qnrB52 / blaTEM-1B / blaCTX-M-14 / cmlA1 / sitABCD iutA / iucD / iucC / iucB / iucA 1..185954 185954
- flank IS/Tn - - 160461..160964 503
- flank IS/Tn - - 167020..167367 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T250255 WP_001159871.1 NZ_CP100121:c162695-162390 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT250255 WP_000813634.1 NZ_CP100121:c162915-162697 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References