Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5353031..5353656 Replicon chromosome
Accession NZ_CP100120
Organism Klebsiella pneumoniae strain GZKP13

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NIK98_RS26230 Protein ID WP_002882817.1
Coordinates 5353031..5353414 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NIK98_RS26235 Protein ID WP_004150355.1
Coordinates 5353414..5353656 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIK98_RS26215 (5350397) 5350397..5351299 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NIK98_RS26220 (5351296) 5351296..5351931 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NIK98_RS26225 (5351928) 5351928..5352857 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NIK98_RS26230 (5353031) 5353031..5353414 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NIK98_RS26235 (5353414) 5353414..5353656 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NIK98_RS26240 (5353861) 5353861..5354778 + 918 WP_004181612.1 alpha/beta hydrolase -
NIK98_RS26245 (5354792) 5354792..5355733 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NIK98_RS26250 (5355778) 5355778..5356215 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NIK98_RS26255 (5356212) 5356212..5357072 - 861 WP_004146232.1 virulence factor BrkB family protein -
NIK98_RS26260 (5357066) 5357066..5357665 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250249 WP_002882817.1 NZ_CP100120:c5353414-5353031 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250249 WP_004150355.1 NZ_CP100120:c5353656-5353414 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References