Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4773662..4774472 Replicon chromosome
Accession NZ_CP100120
Organism Klebsiella pneumoniae strain GZKP13

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NIK98_RS23555 Protein ID WP_004178461.1
Coordinates 4773662..4774195 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NIK98_RS23560 Protein ID WP_002887278.1
Coordinates 4774206..4774472 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIK98_RS23550 (4772493) 4772493..4773614 + 1122 WP_002887282.1 cupin domain-containing protein -
NIK98_RS23555 (4773662) 4773662..4774195 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NIK98_RS23560 (4774206) 4774206..4774472 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NIK98_RS23565 (4774575) 4774575..4776008 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
NIK98_RS23570 (4775998) 4775998..4776681 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NIK98_RS23575 (4776853) 4776853..4778238 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
NIK98_RS23580 (4778256) 4778256..4778600 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T250246 WP_004178461.1 NZ_CP100120:c4774195-4773662 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT250246 WP_002887278.1 NZ_CP100120:c4774472-4774206 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References