Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 78529..79054 Replicon plasmid pHSKP1-2
Accession NZ_CP100116
Organism Klebsiella pneumoniae strain HSKP1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag NIL09_RS27350 Protein ID WP_001159868.1
Coordinates 78529..78834 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag NIL09_RS27355 Protein ID WP_000813634.1
Coordinates 78836..79054 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL09_RS27335 (74439) 74439..75605 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
NIL09_RS27340 (76193) 76193..76948 - 756 WP_000852146.1 replication initiation protein RepE -
NIL09_RS27345 (77722) 77722..78528 - 807 WP_000016982.1 site-specific integrase -
NIL09_RS27350 (78529) 78529..78834 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
NIL09_RS27355 (78836) 78836..79054 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
NIL09_RS27360 (79646) 79646..80134 + 489 WP_011254646.1 hypothetical protein -
NIL09_RS27365 (80168) 80168..81301 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
NIL09_RS27370 (81468) 81468..82241 - 774 WP_000905949.1 hypothetical protein -
NIL09_RS27375 (82254) 82254..82754 - 501 WP_000528931.1 HEPN family nuclease -
NIL09_RS27380 (83019) 83019..83249 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
NIL09_RS27385 (83246) 83246..83662 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / qacE / cmlA1 / sitABCD iutA / iucD / iucC / iucB / iucA 1..110202 110202


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T250232 WP_001159868.1 NZ_CP100116:c78834-78529 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT250232 WP_000813634.1 NZ_CP100116:c79054-78836 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References