250218

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5278561..5279186 Replicon chromosome
Accession NZ_CP100114
Organism Klebsiella pneumoniae strain HSKP1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NIL09_RS25495 Protein ID WP_002882817.1
Coordinates 5278561..5278944 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NIL09_RS25500 Protein ID WP_004150355.1
Coordinates 5278944..5279186 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL09_RS25480 (5275927) 5275927..5276829 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NIL09_RS25485 (5276826) 5276826..5277461 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NIL09_RS25490 (5277458) 5277458..5278387 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NIL09_RS25495 (5278561) 5278561..5278944 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NIL09_RS25500 (5278944) 5278944..5279186 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NIL09_RS25505 (5279391) 5279391..5280308 + 918 WP_004181612.1 alpha/beta hydrolase -
NIL09_RS25510 (5280322) 5280322..5281263 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NIL09_RS25515 (5281308) 5281308..5281745 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NIL09_RS25520 (5281742) 5281742..5282602 - 861 WP_004146232.1 virulence factor BrkB family protein -
NIL09_RS25525 (5282596) 5282596..5283195 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250218 WP_002882817.1 NZ_CP100114:c5278944-5278561 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250218 WP_004150355.1 NZ_CP100114:c5279186-5278944 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References