Detailed information of TA system TA025021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1586864..1587549
• Replicon: chromosome
• Accession: NC_014837
• Organism: Pantoea sp. At-9b

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PAT9B_RS29625
• Protein ID: WP_013508597.1
• Coordinates: 1587373..1587549 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: E6WDB3
• Locus tag: PAT9B_RS07230
• Protein ID: WP_013508596.1
• Coordinates: 1586864..1587268 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAT9B_RS07200	1582951..1583430	+	480	WP_013508592.1	hypothetical protein	-
PAT9B_RS07205	1583563..1583883	+	321	WP_013508593.1	hypothetical protein	-
PAT9B_RS07215	1584532..1584883	+	352	Protein_1395	HNH endonuclease	-
PAT9B_RS07220	1585044..1585517	+	474	WP_013508594.1	phage terminase small subunit P27 family	-
PAT9B_RS07225	1585521..1586336	+	816	Protein_1397	terminase large subunit	-
PAT9B_RS07230	1586864..1587268	-	405	WP_013508596.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PAT9B_RS29625	1587373..1587549	-	177	WP_013508597.1	type II toxin-antitoxin system HicA family toxin	Toxin
PAT9B_RS07235	1588155..1588421	+	267	WP_013508598.1	hypothetical protein	-
PAT9B_RS07240	1588528..1588713	-	186	WP_013508599.1	hypothetical protein	-
PAT9B_RS07245	1588988..1590307	-	1320	WP_013508600.1	TolC family protein	-
PAT9B_RS07250	1590312..1591544	-	1233	WP_013508601.1	HlyD family efflux transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1578339..1593738	15399

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-56)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6728.88 Da
• Isoelectric Point: 10.1493

>T25021 WP_013508597.1 NC_014837:c1587549-1587373 [Pantoea sp. At-9b]
MSSRDFMKLLKERGWILDRIKGSHHVFVKDGKDYHISVPHPEKDLATGTLNKLLKLID

Nucleotide

• Length: 177 bp

>T25021 NC_014837:c1587549-1587373 [Pantoea sp. At-9b]
TTGAGTAGTAGAGACTTTATGAAGCTCCTGAAGGAGCGAGGGTGGATTTTAGACCGTATTAAAGGTAGTCATCATGTTTT
TGTGAAGGATGGTAAAGACTATCACATTTCGGTTCCCCACCCTGAGAAAGACTTAGCGACTGGCACGCTAAATAAGTTGT
TGAAGTTGATAGACTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14686.52 Da
• Isoelectric Point: 4.5295

>AT25021 WP_013508596.1 NC_014837:c1587268-1586864 [Pantoea sp. At-9b]
MKFAVYLHKADSGGYSGFVPDIIGCIFAGDTIDNAIVDATEAIDAHLESLADRGKTLPEAKDISAHLDDEDCQNGIWAYV
DIDVSKYDGKAVKLNITLPQNLLVRIDTFVETHKEFNSRSGFLADLARRELAKA

Nucleotide

• Length: 405 bp

>AT25021 NC_014837:c1587268-1586864 [Pantoea sp. At-9b]
ATGAAATTTGCAGTCTATTTACACAAGGCTGATTCAGGCGGGTATTCTGGCTTCGTCCCAGATATCATTGGCTGCATCTT
TGCCGGTGATACGATAGACAATGCTATCGTTGATGCTACTGAAGCAATTGATGCCCATCTTGAGTCTTTAGCCGATAGAG
GAAAAACGCTTCCCGAAGCGAAAGATATATCAGCTCATCTTGATGATGAAGATTGCCAGAACGGCATCTGGGCTTATGTT
GATATAGACGTAAGCAAATATGATGGCAAGGCTGTAAAGTTAAATATCACATTGCCGCAAAATCTTCTTGTACGCATTGA
CACATTTGTTGAAACACACAAAGAATTTAATTCAAGAAGCGGTTTTTTGGCTGATTTAGCTCGACGCGAGTTAGCTAAAG
CATAA

Structures

Antitoxin

• AlphaFold DB: E6WDB3