Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5326121..5326746 Replicon chromosome
Accession NZ_CP100110
Organism Klebsiella pneumoniae strain HSKP104

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NIL12_RS25760 Protein ID WP_002882817.1
Coordinates 5326121..5326504 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NIL12_RS25765 Protein ID WP_004150355.1
Coordinates 5326504..5326746 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL12_RS25745 (5323487) 5323487..5324389 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NIL12_RS25750 (5324386) 5324386..5325021 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NIL12_RS25755 (5325018) 5325018..5325947 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NIL12_RS25760 (5326121) 5326121..5326504 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NIL12_RS25765 (5326504) 5326504..5326746 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NIL12_RS25770 (5326951) 5326951..5327868 + 918 WP_004181612.1 alpha/beta hydrolase -
NIL12_RS25775 (5327882) 5327882..5328823 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NIL12_RS25780 (5328868) 5328868..5329305 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NIL12_RS25785 (5329302) 5329302..5330162 - 861 WP_004146232.1 virulence factor BrkB family protein -
NIL12_RS25790 (5330156) 5330156..5330755 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250200 WP_002882817.1 NZ_CP100110:c5326504-5326121 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250200 WP_004150355.1 NZ_CP100110:c5326746-5326504 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References